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1. WO2020094828 - MICROBIAL HOSTS ENGINEERED FOR INCREASED TOLERANCE TO TEMPERATURE SHIFTS

Publication Number WO/2020/094828
Publication Date 14.05.2020
International Application No. PCT/EP2019/080645
International Filing Date 08.11.2019
IPC
C12N 9/06 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
06acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
C12N 9/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
C12N 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
CPC
C12N 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
C12N 9/0022
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0012acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
0014acting on the CH-NH2 group of donors (1.4)
0022with oxygen as acceptor (1.4.3)
C12N 9/1077
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
1048Glycosyltransferases (2.4)
1077Pentosyltransferases (2.4.2)
C12N 9/1085
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
1085transferring alkyl or aryl groups other than methyl groups (2.5)
C12Y 104/03016
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
104Oxidoreductases acting on the CH-NH2 group of donors (1.4)
03with oxygen as acceptor (1.4.3)
03016L-Aspartate oxidase (1.4.3.16)
C12Y 204/02019
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
204Glycosyltransferases (2.4)
02Pentosyltransferases (2.4.2)
02019Nicotinate-nucleotide diphosphorylase (carboxylating) (2.4.2.19)
Applicants
  • WAGENINGEN UNIVERSITEIT [NL]/[NL]
Inventors
  • KAMPERS, Linde Francisca Cornelia
  • VOLKERS, Joanna Maria
  • SCHAAP, Petrus Johannes
  • MARTINS DOS SANTOS, Vitor Alexandre Pires
Agents
  • NEDERLANDSCH OCTROOIBUREAU
Priority Data
18205369.409.11.2018EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MICROBIAL HOSTS ENGINEERED FOR INCREASED TOLERANCE TO TEMPERATURE SHIFTS
(FR) HÔTES MICROBIENS MODIFIÉS POUR UNE TOLÉRANCE ACCRUE À DES VARIATIONS DE TEMPÉRATURE
Abstract
(EN)
The present invention relates to microbial host cells that have been engineered for increased tolerance to temperature shifts, for increased performance at temperatures different from the microorganism's optimal temperature and/or for changing at least one of the microorganism's cardinal temperatures by replacing an endogenous NAD+ biosynthesis gene by a heterologous gene encoding a corresponding enzyme with another temperature profile and/or from a microorganism with a different optimum growth temperature.The invention further relates to processes wherein the engineered microbial host cells are used for producing a fermentation product,and to the use nucleotide sequences encoding NAD+ biosynthesis gene for changing at least one of a microorganism's cardinal temperatures and/or for improving a microorganism's tolerance to temperature shifts.
(FR)
La présente invention porte sur des cellules hôtes microbiennes ayant été modifiées pour acquérir une tolérance accrue à des variations de température, pour acquérir une performance accrue à des températures différentes de la température optimale pour un micro-organisme et/ou pour modifier au moins l'une des températures cardinales du micro-organisme en remplaçant un gène de biosynthèse endogène NAD+ par un gène hétérologue codant pour une enzyme correspondante avec un autre profil de température et/ou à partir d'un micro-organisme ayant une température de croissance optimale différente. L'invention concerne en outre des procédés utilisant les cellules hôtes microbiennes modifiées pour produire un produit de fermentation, et l'utilisation de séquences nucléotidiques codant pour le gène de biosynthèse de NAD + pour modifier au moins l'une des températures cardinales du micro-organisme et/ou pour améliorer la tolérance du micro-organisme à des variations de température.
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