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1. WO2020094764 - DOUBLE AND INDUCIBLE SUICIDE GENE CONSTRUCT AND ITS USE IN GENE THERAPY

Publication Number WO/2020/094764
Publication Date 14.05.2020
International Application No. PCT/EP2019/080485
International Filing Date 07.11.2019
IPC
C12N 15/82 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
C12N 9/12 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
C12N 9/64 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50Proteinases
64derived from animal tissue, e.g. rennin
C12N 15/79 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
CPC
C12N 15/85
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 2830/20
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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2830Vector systems having a special element relevant for transcription
20transcription of more than one cistron
C12N 9/1211
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
1211Thymidine kinase (2.7.1.21)
C12N 9/6472
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
48acting on peptide bonds (3.4)
50Proteinases ; , e.g. Endopeptidases (3.4.21-3.4.25)
64derived from animal tissue
6421from mammals
6472Cysteine endopeptidases (3.4.22)
Applicants
  • CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC) [ES]/[ES]
Inventors
  • BACHILLER PÉREZ, Daniel
  • FLEISCHER, Aarne
  • VALLEJO DIEZ, Sara
Agents
  • PONS ARIÑO, Angel
Priority Data
18382792.207.11.2018EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DOUBLE AND INDUCIBLE SUICIDE GENE CONSTRUCT AND ITS USE IN GENE THERAPY
(FR) CONSTRUCTION DE GÈNE SUICIDE DOUBLE ET INDUCTIBLE ET SON UTILISATION EN THÉRAPIE GÉNIQUE
Abstract
(EN)
The present invention provides a double and inducible suicide gene construct that combines the cell death-inducing capacity of the two suicide systems HSV-TK/GCV and iCaspase9/AP20187 in a single polycistronic cassette. The invention also relates to an expression vector comprising said construct, a cell comprising said construct or said vector, pharmaceutical compositions comprising these products and their applications as a medicament for the genetic safety for inducing the specific and directed cell death of specific cell populations that are causing adverse effects in the interior of an organism. The present invention is applicable, for example, in cancer treatments or in transplant situations where the transplanted cells are giving rise to immune rejection or to the formation of tumors.
(FR)
Construction de gène suicide double et inductible combinant la capacité d'induction de mort cellulaire des deux systèmes suicide HSV-TK/GCV et iCaspase9/AP20187 dans une seule cassette polycistronique. L'invention concerne en outre un vecteur d'expression comprenant ladite construction, une cellule comprenant ladite construction ou ledit vecteur, des compositions pharmaceutiques comprenant ces produits et leurs applications en tant que médicament pour la sécurité génétique afin d'induire la mort cellulaire spécifique et dirigée de populations cellulaires spécifiques provoquant des effets indésirables à l'intérieur d'un organisme. La présente invention est applicable, par exemple, à des traitements anticancéreux ou à des situations de transplantation où les cellules transplantées sont responsables du rejet immunitaire ou de la formation de tumeurs.
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