Processing

Please wait...

Settings

Settings

Goto Application

1. WO2020092646 - CELL-FREE RNA LIBRARY PREPARATIONS

Publication Number WO/2020/092646
Publication Date 07.05.2020
International Application No. PCT/US2019/058961
International Filing Date 30.10.2019
IPC
C40B 40/08 2006.01
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40Libraries per se, e.g. arrays, mixtures
04Libraries containing only organic compounds
06Libraries containing nucleotides or polynucleotides, or derivatives thereof
08Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
C12Q 1/6883 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
C40B 40/10 2006.01
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40Libraries per se, e.g. arrays, mixtures
04Libraries containing only organic compounds
10Libraries containing peptides or polypeptides, or derivatives thereof
Applicants
  • MOLECULAR STETHOSCOPE, INC. [US]/[US]
  • SALATHIA, Neeraj [GB]/[US]
Inventors
  • SALATHIA, Neeraj
  • NERENBERG, Michael
  • IBARRA, Arkaitz
  • ZHUANG, Jiali
Agents
  • RIRIE, Ted
Priority Data
62/752,53330.10.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CELL-FREE RNA LIBRARY PREPARATIONS
(FR) PRÉPARATIONS DE BIBLIOTHÈQUE D'ARN ACELLULAIRE
Abstract
(EN)
Diverse cDNA libraries derived from cell-free mRNA and methods of preparing the same are provided. The library may be prepared by extracting RNA from a bodily fluid such as serum or plasma, separating the RNA from contaminants, synthesizing cDNA with a reverse transcriptase enzyme, and enriching protein-coding nucleotide sequences. The library may include a multiplicity of transcripts from solid tissues. Cf-RNA can be measured by qPCR, sequencing, or other suitable methods.
(FR)
L'invention concerne diverses bibliothèques d'ADNc dérivées d'ARNm acellulaire et des procédés de préparation de celles-ci. La bibliothèque peut être préparée en extrayant l'ARN d'un fluide biologique tel que le sérum ou le plasma, en séparant l'ARN des contaminants, en synthétisant l'ADNc avec une enzyme de transcriptase inverse, et en enrichissant des séquences nucléotidiques codant des protéines. La bibliothèque peut comprendre une multiplicité de produits de transcription issus de tissus solides. L'ARN-cf peut être mesuré par qPCR, séquençage, ou autres procédés appropriés.
Latest bibliographic data on file with the International Bureau