Processing

Please wait...

Settings

Settings

Goto Application

1. WO2020091894 - LOW-MACROPHAGE-ADHESION/ACTIVATION CULTURE DEVICES AND METHODS THEREOF FOR CONTINUOUS HEMATOPOIESIS AND EXPANSION OF HEMATOPOIETIC STEM CELLS

Publication Number WO/2020/091894
Publication Date 07.05.2020
International Application No. PCT/US2019/050355
International Filing Date 10.09.2019
IPC
C12M 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
C12M 3/06 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
06with filtration, ultrafiltration, inverse osmosis or dialysis means
C12N 5/09 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
09Tumour cells
CPC
C12M 23/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
23Constructional details, e.g. recesses, hinges
20Material Coatings
C12M 23/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
23Constructional details, e.g. recesses, hinges
34Internal compartments or partitions
C12N 2500/30
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2500Specific components of cell culture medium
30Organic components
C12N 5/0647
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0634Cells from the blood or the immune system
0647Haematopoietic stem cells; Uncommitted or multipotent progenitors
Applicants
  • TSAI, Schickwann [US]/[US]
Inventors
  • TSAI, Schickwann
Priority Data
62/751,69628.10.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) LOW-MACROPHAGE-ADHESION/ACTIVATION CULTURE DEVICES AND METHODS THEREOF FOR CONTINUOUS HEMATOPOIESIS AND EXPANSION OF HEMATOPOIETIC STEM CELLS
(FR) DISPOSITIFS DE CULTURE À FAIBLE ACTIVATION/ADHÉSION DE MACROPHAGES ET PROCÉDÉS ASSOCIÉS POUR L'HÉMATOPOÏÈSE ET L'EXPANSION CONTINUE DE CELLULES SOUCHES HÉMATOPOÏÉTIQUES
Abstract
(EN)
We seek to reduce macrophage adhesion and activation by culturing bone marrow cells in tissue culture devices composed of or covered with polymers with very different protein-binding characteristics than TC-PS such as polyethylene (PE) and other polyolefins, the latter bind proteins via exclusively hydrophobic interactions. As a result, polyolefins bind different proteins and in lower quantities than TC-PS. Furthermore, PE does not contain additional chemical features like the phenolic rings of polystyrene that might contribute to protein binding and macrophage adhesion/activation. Using these new culture devices, we developed a drastically different long-term bone marrow culture, the "Low Macrophage-Adhesion/Activation" (LoMAC) bone marrow culture.
(FR)
L'invention vise à réduire l'adhésion et l'activation des macrophages par la culture de cellules de moelle osseuse dans des dispositifs de culture tissulaire composés ou recouverts de polymères ayant des caractéristiques de liaison aux protéines très différentes de celles des TC-PS telles que le polyéthylène (PE) et d'autres polyoléfines, ces dernières se liant à des protéines par l'intermédiaire d'interactions exclusivement hydrophobes. Par conséquent, les polyoléfines se lient à différentes protéines et en quantités inférieures que les TC-PS. En outre, le PE ne présente pas de caractéristiques chimiques supplémentaires comme les anneaux phénoliques de polystyrène qui pourraient contribuer à la liaison des protéines et à l'adhésion/activation des macrophages. A l'aide de ces nouveaux dispositifs de culture, nous avons développé une culture de moelle osseuse à long terme radicalement différente: la culture de moelle osseuse "à faible adhésion/activation de macrophages" (LoMAC).
Latest bibliographic data on file with the International Bureau