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1. WO2020089487 - CELL CULTURE DEVICE AND METHOD OF USING THE SAME

Publication Number WO/2020/089487
Publication Date 07.05.2020
International Application No. PCT/EP2019/080127
International Filing Date 04.11.2019
IPC
C12M 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
B01L 3/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C12M 3/06 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
06with filtration, ultrafiltration, inverse osmosis or dialysis means
CPC
B01L 2300/0864
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0861Configuration of multiple channels and/or chambers in a single devices
0864comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
B01L 2300/0867
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0861Configuration of multiple channels and/or chambers in a single devices
0867Multiple inlets and one sample wells, e.g. mixing, dilution
B01L 2300/0883
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0861Configuration of multiple channels and/or chambers in a single devices
0883Serpentine channels
B01L 3/502761
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502761specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
C12M 23/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
23Constructional details, e.g. recesses, hinges
02Form or structure of the vessel
16Microfluidic devices; Capillary tubes
C12M 29/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
29Means for introduction, extraction or recirculation of materials, e.g. pumps
10Perfusion
Applicants
  • MICROFLUIDX [GB]/[GB]
Inventors
  • CEJAS, Cesare
  • ALLAF-AKBARI, Elyar
  • ESPINET, Antoine
Agents
  • REGIMBEAU
Priority Data
18306441.902.11.2018EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CELL CULTURE DEVICE AND METHOD OF USING THE SAME
(FR) DISPOSITIF DE CULTURE CELLULAIRE ET MÉTHODE D'UTILISATION CORRESPONDANTE
Abstract
(EN)
The present invention relates to a cell culture device comprising at least a fluidic channel having at least a cell medium inlet and a cell medium outlet, said fluidic channel comprising a lower wall extending between the cell medium inlet and the cell medium outlet, the cell culture device further comprising at least one recess configured to receive a plurality of cells, said recess being formed by the lower wall and defining a bottom surface,andat least a cell trap, said cell trap being configured to capture a cell advected in the fluidic channel and then to make the cell sediment to the bottom surface of the recess.
(FR)
La présente invention porte sur un dispositif de culture cellulaire comprenant au moins un canal fluidique ayant au moins une entrée de milieu cellulaire et une sortie de milieu cellulaire, ledit canal fluidique comprenant une paroi inférieure s'étendant entre l'entrée de milieu cellulaire et la sortie de milieu cellulaire, le dispositif de culture cellulaire comprenant en outre au moins un évidement configuré pour recevoir une pluralité de cellules, ledit évidement étant formé par la paroi inférieure et définissant une surface inférieure, et au moins un piège à cellules, ledit piège à cellules étant configuré pour capturer une cellule advectée dans le canal fluidique puis pour amener le sédiment cellulaire à la surface inférieure de l'évidement.
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