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1. WO2020087989 - BIDIRECTIONAL SUBTRACTION-BASED APTAMER SCREENING METHOD AND KIT

Publication Number WO/2020/087989
Publication Date 07.05.2020
International Application No. PCT/CN2019/096333
International Filing Date 17.07.2019
IPC
C12N 15/115 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
115Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith
CPC
C12N 15/115
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
115Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
C12N 2310/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2310Structure or type of the nucleic acid
10Type of nucleic acid
16Aptamers
C12N 2320/13
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2320Applications; Uses
10in screening processes
13in a process of directed evolution, e.g. SELEX, acquiring a new function
Applicants
  • 廖世奇 LIAO, Shiqi [CN]/[CN]
Inventors
  • 廖世奇 LIAO, Shiqi
  • 袁红霞 YUAN, Hongxia
  • 栗怡 LI, Yi
Agents
  • 深圳市尔逊专利代理事务所(普通合伙) SHENZHEN ELSON PATENT AGENT FIRM (GENERAL PARTNERSHIP)
Priority Data
201811278330.130.10.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) BIDIRECTIONAL SUBTRACTION-BASED APTAMER SCREENING METHOD AND KIT
(FR) PROCÉDÉ ET KIT DE CRIBLAGE D'APTAMÈRES À BASE DE SOUSTRACTION BIDIRECTIONNELLE
(ZH) 双向消减核酸适配体筛选方法及试剂盒
Abstract
(EN)
A bidirectional thermal cycle subtraction SELEX-based rapid aptamer screening method. According to the method, a nano magnetic bead material, SELEX technology, and high-throughput nucleic acid sequencing are organically combined, and rapid and efficient aptamer screening technical systems and corresponding kits aiming at different targets are established. The kit can achieve rapid screening of an aptamer having strong specificity and high affinity. The method has the advantages of being rapid, efficient, low in costs, easy to operate, capable of being mechanically completed, and the like.
(FR)
L'invention concerne un procédé de criblage d'aptamères rapide basé sur le SELEX par soustraction de cycle thermique bidirectionnel. Selon le procédé, un matériau de bille magnétique nanométrique, la technologie SELEX et un séquençage d'acide nucléique à haut débit sont combinés organiquement et des systèmes techniques de criblage d'aptamères rapides et efficaces ainsi que des kits correspondants visant des cibles différentes sont établis. Le kit peut permettre un criblage rapide d'un aptamère ayant une forte spécificité et une affinité élevée. Le procédé présente les avantages d'être rapide, efficace, de faible coût, facile à utiliser, pouvant être complété mécaniquement et analogues.
(ZH)
一种双向热循环消减SELEX快速筛选核酸适配体的方法,该方法将纳米磁珠材料、SELEX技术和高通量核酸测序有机结合,建立针对不同靶标的快速高效核酸适配体筛选技术体系及相应试剂盒。该试剂盒可快速筛选出特异性强、亲和力高的核酸适配体。该方法具有快速、高效、成本低、操作简单、可机械化完成等优点。
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Latest bibliographic data on file with the International Bureau