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1. WO2020087966 - THREE-DIMENSIONAL DIFFRACTION TOMOGRAPHY MICROSCOPIC IMAGING METHOD BASED ON LED ARRAY CODING ILLUMINATION

Publication Number WO/2020/087966
Publication Date 07.05.2020
International Application No. PCT/CN2019/094886
International Filing Date 05.07.2019
IPC
G01N 15/14 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation
CPC
G01N 15/1434
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1434using an analyser being characterised by its optical arrangement
G01N 15/1468
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1468with spatial resolution of the texture or inner structure of the particle
G01N 2015/1445
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1434using an analyser being characterised by its optical arrangement
144Imaging characterised by its optical setup
1445Three-dimensional imaging, imaging in different image planes, e.g. under different angles or at different depths, e.g. by a relative motion of sample and detector, for instance by tomography
G01N 2015/145
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1434using an analyser being characterised by its optical arrangement
1447Spatial selection
145by pattern of light, e.g. fringe pattern
Applicants
  • 南京理工大学 NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY [CN]/[CN]
Inventors
  • 左超 ZUO, Chao
  • 陈钱 CHEN, Qian
  • 孙佳嵩 SUN, Jiasong
  • 张玉珍 ZHANG, Yuzhen
  • 顾国华 GU, Guohua
Agents
  • 江苏圣典律师事务所 JIANGSU SHENGDIAN LAW FIRM
Priority Data
201811292260.501.11.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) THREE-DIMENSIONAL DIFFRACTION TOMOGRAPHY MICROSCOPIC IMAGING METHOD BASED ON LED ARRAY CODING ILLUMINATION
(FR) PROCÉDÉ D'IMAGERIE MICROSCOPIQUE PAR TOMOGRAPHIE PAR DIFFRACTION TRIDIMENSIONNELLE BASÉ SUR UN ÉCLAIRAGE À CODAGE DE RÉSEAU DE DEL
(ZH) 基于LED阵列编码照明的三维衍射层析显微成像方法
Abstract
(EN)
Disclosed is a three-dimensional diffraction tomography microscopic imaging method based on LED array coding illumination. The method comprises: firstly acquiring an original intensity image; acquiring three groups of intensity image stacks in different defocusing positions by means of moving an objective table or using an electrically controlled zoom lens; then, deducing a three-dimensional phase transfer function of a microscopic imaging system in any shape illumination by means of acquiring intensity image stacks of an object to be tested in the different defocusing positions to obtain a three-dimensional phase transfer function of the microscopic system in circular and annular illumination at different correlation coefficients, and performing deconvolution reconstruction on a three-dimensional diffraction tomography quantitative refractive index; performing an inverse Fourier transform on a three-dimensional scattering potential function; and converting the scattering potential function into refractive index distribution so that the quantitative three-dimensional refractive index distribution of the tested object can be obtained. The high resolution and high signal-to-noise ratio three-dimensional diffraction tomography microscopic imaging of samples, such as cells and tiny biological tissues, is realized.
(FR)
L'invention concerne un procédé d'imagerie microscopique par tomographie par diffraction tridimensionnelle basé sur un éclairage à codage de réseau de DEL. Le procédé consiste à : tout d'abord acquérir une image d'intensité d'origine ; acquérir trois groupes d'empilements d'images d'intensité dans différentes positions de défocalisation au moyen du déplacement d'une table d'objectif ou à l'aide d'une lentille zoom commandée électriquement ; puis, déduire une fonction de transfert de phase tridimensionnelle d'un système d'imagerie microscopique dans n'importe quel éclairage de forme au moyen de l'acquisition d'empilements d'images d'intensité d'un objet à tester dans les différentes positions de défocalisation pour obtenir une fonction de transfert de phase tridimensionnelle du système microscopique dans un éclairage circulaire et annulaire à différents coefficients de corrélation et réaliser une reconstruction par déconvolution sur un indice de réfraction quantitatif de tomographie par diffraction tridimensionnelle ; réaliser une transformée de Fourier inverse sur une fonction de potentiel de diffusion tridimensionnelle ; et convertir la fonction de potentiel de diffusion en une distribution d'indice de réfraction de sorte que la distribution quantitative d'indice de réfraction tridimensionnel de l'objet testé puisse être obtenue. L'imagerie microscopique par tomographie par diffraction tridimensionnelle à rapport signal sur bruit élevé et à haute résolution d'échantillons, tels que des cellules et de minuscules tissus biologiques, est réalisée.
(ZH)
一种基于LED阵列编码照明的三维衍射层析显微成像方法,首先采集原始强度图像,通过移动载物台或利用电控变焦透镜采集在不同离焦位置下的三组强度图像堆栈,然后通过采集待测物体在不同离焦位置下的强度图像堆栈,对任意形状照明的显微成像系统的三维相位传递函数进行推导,得到在不同相干系数下的圆形和环状照明下显微系统的三维相位传递函数,并对三维衍射层析定量折射率反卷积重构,对三维散射势函数进行逆傅里叶变换,将散射势函数转换为折射率分布,即可得到被测物体的定量三维折射率分布。实现了对细胞、微小生物组织等样品高分辨率高信噪比三维衍射层析显微成像。
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