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1. WO2020087113 - METHODS, REAGENTS AND KITS FOR DETECTION OF KARNAL BUNT

Publication Number WO/2020/087113
Publication Date 07.05.2020
International Application No. PCT/AU2019/051185
International Filing Date 29.10.2019
IPC
C12Q 1/6895 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
6895for plants, fungi or algae
C12Q 1/6844 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/6865 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
6865Promoter-based amplification, e.g. nucleic acid sequence-based amplification , self-sustained sequence replication or transcription-based amplification system
CPC
A01N 1/0221
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
0205Chemical aspects
021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
0221Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
A01N 25/00
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
25Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, ; e.g. seed treatment or sequential application;
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/6895
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
6895for plants, fungi or algae
C12Q 2600/118
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
118Prognosis of disease development
G01N 2333/37
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
37from fungi
Applicants
  • THE CROWN IN RIGHT OF THE STATE OF NEW SOUTH WALES ACTING THROUGH THE DEPARTMENT OF PRIMARY INDUSTRIES AS AN OFFICE OF THE DEPARTMENT OF INDUSTRY [AU]/[AU]
Inventors
  • TAN, Mui-Keng
Agents
  • FB RICE
Priority Data
201890410129.10.2018AU
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS, REAGENTS AND KITS FOR DETECTION OF KARNAL BUNT
(FR) PROCÉDÉS, RÉACTIFS ET KITS POUR LA DÉTECTION DE CARIE DE KARNAL
Abstract
(EN)
The present disclosure relates to methods and test kits for detecting Tilletia indica (Karnal bunt) in biological samples. In particular, the present disclosure relates to a diagnostic method employing isothermal amplification of nucleic acids, namely a loop-mediated amplification (LAMP), in combination with lateral flow technology to detect Karnal bunt in a grain sample, e.g., from wheat or triticale, as well as test kits and reagent mixtures for use in same.
(FR)
La présente invention concerne des procédés et des nécessaires de test pour détecter Tilletia indica (carie de Karnal) dans des échantillons biologiques. En particulier, la présente invention concerne un procédé de diagnostic utilisant une amplification isotherme d'acides nucléiques, à savoir une amplification à médiation par boucle (LAMP), en combinaison avec une technologie de flux latéral pour détecter la carie de Karnal dans un échantillon de grain, par exemple, de blé ou de triticale, ainsi que des nécessaires de test et des mélanges de réactifs destinés à être utilisés dans ceux-ci.
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