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1. WO2020069603 - A MICRO-PIPETTE TIP FOR FORMING MICRO-DROPLETS

Publication Number WO/2020/069603
Publication Date 09.04.2020
International Application No. PCT/CA2019/051330
International Filing Date 19.09.2019
IPC
C12M 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
B01F 15/02 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
15Accessories for mixers
02Feed or discharge mechanisms
B01F 3/08 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
3Mixing, e.g. dispersing, emulsifying, according to the phases to be mixed
08liquids with liquids; Emulsifying
B01L 3/02 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
02Burettes; Pipettes
C12M 1/38 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
36including condition or time responsive control, e.g. automatically controlled fermentors
38Temperature-responsive control
C12Q 1/686 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction
CPC
B01L 2200/16
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
16Reagents, handling or storing thereof
B01L 2300/165
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
16Surface properties and coatings
161Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
165Specific details about hydrophobic, oleophobic surfaces
B01L 3/021
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
02Burettes; Pipettes
021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
Applicants
  • S.M. RESEARCH INC. [CA]/[CA]
Inventors
  • XIAO, Lifeng
  • WU, Yuan Min
  • CHEN, Yuanji
Agents
  • ABM INTELLECTUAL PROPERTY INC.
Priority Data
16/148,28201.10.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) A MICRO-PIPETTE TIP FOR FORMING MICRO-DROPLETS
(FR) POINTE DE MICRO-PIPETTE POUR FORMER DES MICRO-GOUTTELETTES
Abstract
(EN)
A micro-droplet-emulsifier to generate a micro-droplet-emulsion for application on digital polymerase chain reaction is provided. This device comprises of a micro-pipette to hold a dispersed-phase-liquid; a droplet generator that attaches to the micro-pipette and has a plurality of substantially flat micro-channels. The dispersed-phase-liquid is forced through the micro-channels to form micro-droplet-emulsion of dispersed-liquid-phase in the continuous-liquid-phase inside the chamber. The size of the micro-droplets is controlled by the shape and the aspect ratio of the micro-channels, the depth of micro-channel and the material of the micro-droplet-generator-head that dictates the contact angle of the droplet on the micro-channels.
(FR)
Micro-émulsifiant à micro-gouttelettes servant à produire une émulsion de micro-gouttelettes destinée à être appliquée sur une réaction en chaîne par polymérase numérique. Ce dispositif comprend une micro-pipette pour contenir un liquide à phase dispersée; un producteur de gouttelettes qui se fixe à la micro-pipette et comporte une pluralité de micro-canaux pratiquement plats. Le liquide à phase dispersée est contraint à passer à travers les microcanaux pour former une émulsion de micro-gouttelettes à phase liquide dispersée dans la phase liquide continue à l'intérieur de la chambre. La taille des micro-gouttelettes est régulée par la forme et le rapport d'aspect des micro-canaux, la profondeur du micro-canal et le matériau de la tête du producteur de micro-gouttelettes qui dicte l'angle de contact de la gouttelette sur les micro-canaux.
Also published as
Latest bibliographic data on file with the International Bureau