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1. WO2020068559 - DEPLETING UNWANTED RNA SPECIES

Publication Number WO/2020/068559
Publication Date 02.04.2020
International Application No. PCT/US2019/051999
International Filing Date 19.09.2019
IPC
C12Q 1/6848 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
6848characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
CPC
C12Q 1/6848
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6848characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Applicants
  • QIAGEN SCIENCES, LLC [US]/[US]
Inventors
  • SHAFFER, Jonathan
  • LADER, Eric
  • TOLSTRUP, Niels
  • KRUMMHEUER, Joerg
  • KIM, Daniel Y.
  • STRAUSS, Sascha
Agents
  • LIN, Qing
  • OBEIDAT, Baha, A.
  • KUMABE, Blake, K.
  • SOLTANI, Bobby, B.
  • ZENTZ, Bradley, J.
  • SARGEANT, Brooke
  • QUIST, Brooke, W.
  • ROTH, Carol, J.
  • EIDT, Chandra, E.
  • WITTKOPP, Cristina, J.
  • O'BRIEN, Daniel
  • CARLSON, David, V.
  • STARK, Duncan
  • TARLETON, E., Russell
  • SUN, Eileen, S.
  • HARWOOD, Eric, A.
  • CHAN, Frieda, K.
  • GERTZ, Glenda, A.
  • HAN, Hai
  • TALBERT, Hayley, J.
  • BARRETT, Jared, M.
  • PEPE, Jeffrey, C.
  • SAKOI, Jeffrey, M.
  • BAUNACH, Jeremiah, J.
  • ZHANG, Jianping
  • MORGAN, John, A.
  • WAKELEY, John, J.
  • COE, Justin, E.
  • HENCKEL, Karen, M.
  • HEFTER, Karl, A.
  • HERMANNS, Karl, R.
  • MORGAN, Kevan, L.
  • COSTANZA, Kevin, S.
  • COOPER, Michael, P.
  • RUSYN, Paul
  • HALLER, Rachel, A.
  • IANNUCCI, Robert
  • WEBB, Samuel, E.
  • LEEK, Shoko, I.
  • ROSENMAN, Stephen, J.
  • ABEDI, Syed
  • BOLLER, Timothy, L.
  • LIGON, Toby, J.
  • SHEWMAKE, Thomas, A.
  • CANTRELL, Tyler, C.
  • CLAYTON, Kenneth W.
  • SAKOI, Zachary M.
Priority Data
62/736,00625.09.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DEPLETING UNWANTED RNA SPECIES
(FR) APPAUVRISSEMENT EN ESPÈCES D'ARN INDÉSIRABLES
Abstract
(EN)
The present disclosure provides methods and kits for inhibiting cDNA synthesis of unwanted RNA species during reverse transcription. The methods and kits provided herein use blocking oligonucleotides such as those comprising locked nucleic acids (LNAs).
(FR)
La présente invention concerne des procédés et des kits pour inhiber la synthèse d'ADNc d'espèces d'ARN indésirables pendant la transcription inverse. Les procédés et les kits selon l'invention utilisent des oligonucléotides de blocage tels que ceux comprenant des acides nucléiques verrouillés (LNA).
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