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1. WO2020068014 - TETRAHYMENA THERMOPHILA ARTIFICIAL CHROMOSOME 1 (TTAC1) AND ITS USE FOR THE PRODUCTION OF RECOMBINANT PROTEINS

Publication Number WO/2020/068014
Publication Date 02.04.2020
International Application No. PCT/TR2019/050498
International Filing Date 24.06.2019
CPC
C12N 15/79
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
C12P 21/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
Applicants
  • ANADOLU UNIVERSITESI [TR]/[TR]
Inventors
  • ARSLANYOLU, Muhittin
  • ÜSTÜNTANIR DEDE, Ayca Fulya
Agents
  • INVOKAT INTELLECTUAL PROPERTY SERVICES
Priority Data
2018/1388025.09.2018TR
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) TETRAHYMENA THERMOPHILA ARTIFICIAL CHROMOSOME 1 (TTAC1) AND ITS USE FOR THE PRODUCTION OF RECOMBINANT PROTEINS
(FR) CHROMOSOME ARTIFICIEL 1 DE TETRAHYMENA THERMOPHILA (TTAC1) ET SON UTILISATION POUR LA PRODUCTION DE PROTÉINES RECOMBINANTES
Abstract
(EN)
The present invention relates to a Tetrahymena thermophila artificial chromosome 1 comprising a telomere sequence having SEQ ID NO: 1. In more preferred embodiments, the artificial chromosome of the present invention further comprises a C3 replicative origin "Tt rDNA C3 ori" (SEQ ID NO: 2), a modified HSP70.2 promoter (SEQ ID NO: 3), a PLA signal sequence (SEQ ID NO: 5), and a sfGFP marker gene (SEQ ID NO: 6) to obtain an artificial chromosome in a circular or linear form. In further aspects, the present invention relates to a novel method for obtaining said artificial chromosome, and utilization of it for producing recombinant proteins.
(FR)
La présente invention concerne un chromosome artificiel 1 de Tetrahymena thermophila comprenant une séquence télomérique ayant SEQ ID NO: 1. Dans des modes de réalisation plus préférés, le chromosome artificiel de la présente invention comprend en outre une origine réplicative en C3 « ori C3 ADNr Tt » (SEQ ID NO: 2), un promoteur HSP70.2 modifié (SEQ ID NO:3), une séquence de signal PLA (SEQ ID NO: 5), et un gène marqueur de sfGFP (SEQ ID NO: 6) afin d'obtenir un chromosome artificiel sous une forme circulaire ou linéaire. Selon d'autres aspects, la présente invention concerne un nouveau procédé d'obtention dudit chromosome artificiel, et son utilisation pour la production de protéines recombinantes.
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