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1. WO2020067502 - CULTURE ADDITIVE, CULTURE MEDIUM AND CULTURE METHOD FOR ANIMAL CELLS

Publication Number WO/2020/067502
Publication Date 02.04.2020
International Application No. PCT/JP2019/038354
International Filing Date 27.09.2019
IPC
C12N 5/07 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
CPC
C12N 2500/32
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2500Specific components of cell culture medium
30Organic components
32Amino acids
C12N 2500/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2500Specific components of cell culture medium
30Organic components
34Sugars
C12N 5/0031
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0018Culture media for cell or tissue culture
0031Serum-free culture media
C12N 5/0696
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0696Artificially induced pluripotent stem cells, e.g. iPS
Applicants
  • 味の素株式会社 AJINOMOTO CO., INC. [JP]/[JP]
Inventors
  • 松本 拓也 MATSUMOTO, Takuya
  • 小川 晋平 OGAWA, Shimpei
  • 風呂光 俊平 FUROMITSU, Shumpei
  • 大矢 裕介 OHYA, Yusuke
  • 古関 琴衣 KOSEKI, Kotoe
  • 樋口 拓哉 HIGUCHI, Takuya
  • 西山 恵 NISHIYAMA, Megumi
Agents
  • 高島 一 TAKASHIMA, Hajime
Priority Data
2018-18435228.09.2018JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) CULTURE ADDITIVE, CULTURE MEDIUM AND CULTURE METHOD FOR ANIMAL CELLS
(FR) ADDITIF DE CULTURE, MILIEU DE CULTURE ET PROCÉDÉ DE CULTURE POUR CELLULES ANIMALES
(JA) 動物細胞の培養用添加物、培養用培地および培養方法
Abstract
(EN)
The present invention pertains to: a culture additive for animal cells, said culture additive containing an amino acid and/or glucose; a culture medium for animal cells, said culture medium containing an amino acid and/or glucose in addition to medium component(s); and a culture method for animal cells, said method comprising culturing animal cells with the use of the medium or adding the additive to a culture. According to the present invention, the proliferation ability of animal cells can be improved particularly in the case where the animal cells are cultured using a serum-free medium or a low-albumin medium.
(FR)
La présente invention concerne : un additif de culture pour des cellules animales, ledit additif de culture contenant un acide aminé et/ou du glucose ; un milieu de culture pour des cellules animales, ledit milieu de culture contenant un acide aminé et/ou du glucose en plus du (des) composant(s) de milieu ; et un procédé de culture pour cellules animales, ledit procédé comprenant la culture de cellules animales en utilisant le milieu ou en ajoutant l'additif à une culture. Selon la présente invention, la capacité de prolifération de cellules animales peut être améliorée en particulier dans le cas où les cellules animales sont cultivées à l'aide d'un milieu dépourvu de sérum ou d'un milieu à faible teneur en albumine.
(JA)
本発明は、アミノ酸もしくはグルコース、またはアミノ酸およびグルコースを含有する動物細胞の培養用添加物、培地成分に加えて、アミノ酸もしくはグルコース、またはアミノ酸およびグルコースをさらに含有する動物細胞の培養用培地、ならびに前記培地を用いて動物細胞を培養すること、あるいは前記添加物を培養物に添加することを含む、動物細胞の培養方法に関する。 本発明によれば、特に無血清培地や低アルブミン培地を用いて動物細胞の培養を行う際に、動物細胞の増殖性を向上させることができる。
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