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1. WO2020066722 - HEMOGLOBIN ASSAY REAGENT, ASSAY KIT AND ASSAY METHOD

Publication Number WO/2020/066722
Publication Date 02.04.2020
International Application No. PCT/JP2019/036235
International Filing Date 13.09.2019
IPC
G01N 33/545 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
544the carrier being organic
545Synthetic resin
G01N 33/53 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 33/543 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
C07K 16/18 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
CPC
C07K 16/18
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
G01N 33/53
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 33/543
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
G01N 33/545
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
544the carrier being organic
545Synthetic resin
Applicants
  • 栄研化学株式会社 EIKEN KAGAKU KABUSHIKI KAISHA [JP]/[JP]
Inventors
  • 油井 恵 YUI Megumi
  • 牧之段 満 MAKINODAN Mitsuru
Agents
  • 長谷川 芳樹 HASEGAWA Yoshiki
  • 清水 義憲 SHIMIZU Yoshinori
  • 木元 克輔 KIMOTO Kosuke
Priority Data
2018-17995226.09.2018JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) HEMOGLOBIN ASSAY REAGENT, ASSAY KIT AND ASSAY METHOD
(FR) RÉACTIF DE DOSAGE DE L'HÉMOGLOBINE, KIT DE DOSAGE ET PROCÉDÉ DE DOSAGE
(JA) ヘモグロビンの測定試薬、測定キット及び測定方法
Abstract
(EN)
The present invention addresses the problem of providing a hemoglobin assay reagent and assay method that allow for more accurate measurement of the amount of hemoglobin by suppressing a reduction in a hemoglobin measurement value triggered by the addition of haptoglobin. This hemoglobin assay reagent contains: an insoluble carrier that supports an anti-hemoglobin antibody; and an insoluble carrier that supports an anti-haptoglobin antibody. This method for measuring hemoglobin in a specimen comprises: (1) a step in which a specimen including a hemoglobin-haptoglobin complex is obtained by mixing together a specimen and haptoglobin to cause a hemoglobin-haptoglobin complex to be formed; and (2) a step in which the specimen obtained in (1) is brought into contact with the insoluble carrier that supports an anti-hemoglobin antibody and the insoluble carrier that supports an anti-haptoglobin antibody to cause immunoagglutination.
(FR)
La présente invention aborde le problème consistant à fournir un réactif de dosage de l'hémoglobine et un procédé de dosage qui permettent de mesurer de manière plus précise la quantité d'hémoglobine en supprimant une baisse d'une valeur de mesure d'hémoglobine déclenchée par l'ajout d'haptoglobine. Ce réactif de dosage de l'hémoglobine contient : un vecteur insoluble qui supporte un anticorps anti-hémoglobine ; et un véhicule insoluble qui supporte un anticorps anti-haptoglobine. Ce procédé de mesure de l'hémoglobine dans un échantillon comprend : (1) une étape dans laquelle un échantillon comprenant un complexe hémoglobine-haptoglobine est obtenu en mélangeant ensemble un échantillon et de l'haptoglobine afin de provoquer la formation d'un complexe hémoglobine-haptoglobine ; et (2) une étape dans laquelle l'échantillon obtenu en (1) est mis en contact avec le véhicule insoluble qui supporte un anticorps anti-hémoglobine et le véhicule insoluble qui supporte un anticorps anti-haptoglobine pour provoquer une immunoagglutination.
(JA)
本発明の課題はハプトグロビンの添加に起因するヘモグロビンの測定値の低下を抑制して、ヘモグロビンの量をより正確に測定できるヘモグロビンの測定試薬及び測定方法を提供することである。本発明のヘモグロビンを測定するための試薬は、抗ヘモグロビン抗体を担持する不溶性担体と、抗ハプトグロビン抗体を担持する不溶性担体とを含む。本発明の検体中のヘモグロビンを測定するための方法は、(1)検体とハプトグロビンとを混合し、ヘモグロビン-ハプトグロビン複合体を形成させ、ヘモグロビン-ハプトグロビン複合体を含む試料を得る工程と、(2)工程(1)で得られた試料と、抗ヘモグロビン抗体を担持する不溶性担体、及び抗ハプトグロビン抗体を担持する不溶性担体とを接触させ、免疫凝集させる工程と、を含む。
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