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1. WO2020064793 - METHODS AND USES FOR DETERMINING OSTEOGENIC POTENTIAL OF IN VITRO DIFFERENTIATED CELLS

Publication Number WO/2020/064793
Publication Date 02.04.2020
International Application No. PCT/EP2019/075794
International Filing Date 25.09.2019
IPC
C12Q 1/6881 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6881for tissue or cell typing, e.g. human leukocyte antigen probes
C12Q 1/6876 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12N 5/077 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C12N 5/0775 2010.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775Mesenchymal stem cells; Adipose-tissue derived stem cells
CPC
C12N 5/0654
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0652Cells of skeletal and connective tissues; Mesenchyme
0654Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
C12Q 1/6876
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12Q 1/6881
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6881for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
C12Q 2600/158
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
158Expression markers
Applicants
  • BONE THERAPEUTICS SA [BE]/[BE]
Inventors
  • PIETRI, Sandra
  • DE TROY, Delphine
  • TRUS, Caroline
  • NORMAND, Sylvain
  • HERTZOG, Laure
Agents
  • DE CLERCQ & PARTNERS
Priority Data
18196721.725.09.2018EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND USES FOR DETERMINING OSTEOGENIC POTENTIAL OF IN VITRO DIFFERENTIATED CELLS
(FR) PROCÉDÉS ET UTILISATIONS ASSOCIÉES POUR LA DÉTERMINATION DU POTENTIEL OSTÉOGÉNIQUE DE CELLULES DIFFÉRENCIÉES IN VITRO
Abstract
(EN)
The application provides the use of CD73, CD105, CD44 and/or CD10 for determining osteogenic potential of in vitro differentiated cells. The application further provides a method for determining osteogenic potential of in vitro differentiated cells comprising measuring the quantity of the in vitro differentiated cells expressing CD73, CD105, CD10 and/or CD44, and/or measuring the quantity of CD73, CD105 and/or CD44 expressed by the in vitro differentiated cells. The invention also provides a method for selecting a subject for preparing in vitro differentiated cells of chondro-osteoblastic lineage comprising recovering MSC from a biological sample of a subject; obtaining in vitro differentiated cells from the MSC; determining the osteogenic potential of the in vitro differentiated cells by a method as disclosed herein; and selecting the subject for preparing in vitro differentiated cells of chondro-osteoblastic lineage if the in vitro differentiated cells have clinically useful osteogenic potential.
(FR)
L'invention concerne l'utilisation de CD73, CD105, CD44 et/ou CD10 pour déterminer le potentiel ostéogénique de cellules différenciées in vitro. L'invention concerne en outre un procédé de détermination du potentiel ostéogénique de cellules différenciées in vitro comprenant la mesure de la quantité des cellules différenciées in vitro exprimant CD73, CD105, CD10 et/ou CD44, et/ou la mesure de la quantité de CD73, CD105 et/ou CD44 exprimée par les cellules différenciées in vitro. L'invention concerne également un procédé de sélection d'un sujet pour préparer des cellules différenciées in vitro de lignée chondro-ostéoblastique comprenant la récupération de CSM à partir d'un échantillon biologique d'un sujet ; l'obtention de cellules différenciées in vitro à partir de CSM ; la détermination du potentiel ostéogénique des cellules différenciées in vitro par un procédé selon l'invention ; et la sélection du sujet pour la préparation de cellules différenciées in vitro de lignée chondro-ostéoblastique si les cellules différenciées in vitro ont un potentiel ostéogénique cliniquement utile.
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