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1. WO2020063667 - SOLUTION CHANGING METHOD, SOLUTION CHANGING PLATE, AND USES THEREOF IN CELL STAINING AND PARTICLE WASHING

Publication Number WO/2020/063667
Publication Date 02.04.2020
International Application No. PCT/CN2019/107804
International Filing Date 25.09.2019
IPC
G01N 35/00 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
B01L 3/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
CPC
B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
G01N 33/50
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N 35/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
Applicants
  • 重庆微浪生物科技有限公司 CHONGQING WAVELET BIOLOGICAL TECHNOLOGY CO., LTD. [CN]/[CN]
Inventors
  • 万瑛 WAN, Ying
  • 王建 WANG, Jian
  • 周见至 ZHOU, Jianzhi
  • 倪青山 NI, Qingshan
  • 邹丽云 ZOU, Liyun
  • 李全滨 LI, Quanbin
  • 王晓玲 WANG, Xiaoling
Agents
  • 上海光华专利事务所(普通合伙) J.Z.M.C. PATENT AND TRADEMARK LAW OFFICE (GENERAL PARTNERSHIP)
Priority Data
201811127633.327.09.2018CN
201821575639.227.09.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) SOLUTION CHANGING METHOD, SOLUTION CHANGING PLATE, AND USES THEREOF IN CELL STAINING AND PARTICLE WASHING
(FR) PROCÉDÉ DE CHANGEMENT DE SOLUTION, PLAQUE DE CHANGEMENT DE SOLUTION ET UTILISATIONS ASSOCIÉES DANS LA COLORATION DE CELLULES ET LE LAVAGE DE PARTICULES
(ZH) 一种换液方法、换液板及其在细胞染色和颗粒洗涤中的用途
Abstract
(EN)
A solution changing method, a solution changing plate, and uses thereof in cell staining and particle washing. The solution changing method comprises the following steps: on a solution changing plate, injecting a mixed cell suspension or particle suspension into at least one sample tank (21) and allowing to stand still, thus allowing the cells in the mixed cell suspension or the particles in the particle suspension to settle; continuously injecting a buffer solution into a first cleaning solution tank (22) and a second cleaning solution tank (24) connected to the sample tank (21), and continuously discharging the buffer solution from the second cleaning solution tank (24) and the first cleaning solution tank (22).
(FR)
L'invention concerne un procédé de changement de solution, une plaque de changement de solution, et les utilisations associées dans la coloration de cellules et le lavage de particules. Le procédé de changement de solution comprend les étapes suivantes : sur une plaque de changement de solution, injecter une suspension de cellules mélangées ou une suspension de particules dans au moins un réservoir d'échantillon (21) et laisser reposer, permettant ainsi aux cellules de la suspension de cellules mélangées ou aux particules dans la suspension de particules de se déposer; injecter en continu une solution tampon dans un premier réservoir de solution de nettoyage (22) et un second réservoir de solution de nettoyage (24) raccordé au réservoir d'échantillon (21), et décharger en continu la solution tampon du second réservoir de solution de nettoyage (24) et du premier réservoir de solution de nettoyage (22).
(ZH)
一种换液方法、换液板及其在细胞染色和颗粒洗涤中的用途,其中换液方法包括如下步骤,在换液板上,向至少一个样本槽(21)中注入混合细胞悬液或颗粒悬液并静置,使混合细胞悬液中的细胞或颗粒悬液中的颗粒沉降;向与样本槽(21)连接的第一洗液槽(22)和第二洗液槽(24)中连续注入缓冲液并从第二洗液槽(24)和第一洗液槽(22)中连续排出缓冲液。
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