Processing

Please wait...

Settings

Settings

Goto Application

1. WO2020063161 - METHOD FOR EXPANDING HEPATOCYTE IN VITRO AND APPLICATION

Publication Number WO/2020/063161
Publication Date 02.04.2020
International Application No. PCT/CN2019/100511
International Filing Date 14.08.2019
IPC
C12N 5/071 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
C12N 5/074 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
074Adult stem cells
A61K 35/407 2015.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
37Digestive system
407Liver; Hepatocytes
A61P 1/16 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
1Drugs for disorders of the alimentary tract or the digestive system
16for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
CPC
A61K 35/407
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
37Digestive system
407Liver; Hepatocytes
A61P 1/16
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
1Drugs for disorders of the alimentary tract or the digestive system
16for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
C12Q 1/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
Applicants
  • 中国科学院分子细胞科学卓越创新中心 CENTER FOR EXCELLENCE IN MOLECULAR CELL SCIENCE, CHINESE ACADEMY OF SCIENCES [CN]/[CN]
Inventors
  • 惠利健 HUI, Lijian
  • 张鲁狄 ZHANG, Ludi
  • 张坤 ZHANG, Kun
Agents
  • 上海专利商标事务所有限公司 SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC
Priority Data
201811156216.130.09.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR EXPANDING HEPATOCYTE IN VITRO AND APPLICATION
(FR) PROCÉDÉ DE MULTIPLICATION D'UN HÉPATOCYTE IN VITRO APPLICATION CORRESPONDANTE
(ZH) 肝细胞的体外扩增培养方法及应用
Abstract
(EN)
Provided are a method for expanding a hepatocyte in vitro and an application. A culture system of a reprogrammed human hepatocyte is provided to serve as a proliferable intermediate cell between a mature hepatocyte and a hepatic progenitor cell. The liver repopulation ability of the hepatocyte was verified in animals. The method does not require the introduction of an exogenous gene into a hepatocyte, and the expansion of the hepatocyte can be realized by means of a conventional culture. The obtained hepatocyte is passable; and can be cultured to maturation to obtain a functional mature human hepatocyte.
(FR)
Procédé de multiplication d'un hépatocyte in vitro et application correspondante Un système de culture d'un hépatocyte humain reprogrammé est utilisé pour servir de cellule intermédiaire capable de proliférer entre un hépatocyte mature et une cellule progénitrice hépatique. La capacité de repopulation du foie par l'hépatocyte a été démontrée chez les animaux. Le procédé ne nécessite pas l'introduction d'un gène exogène dans un hépatocyte, et la multiplication de l'hépatocyte peut être réalisée au moyen d'une culture classique. L'hépatocyte obtenu est acceptable; et peut être cultivé jusqu'à maturation pour obtenir un hépatocyte humain mature fonctionnel.
(ZH)
提供一种肝细胞的体外扩增培养方法及应用,提供了一种重编程人肝细胞为可增殖的处于成熟肝细胞和肝前体细胞之间的中间态细胞的培养体系,并在动物体内验证其肝脏再殖能力。所述方法不需要在肝细胞内导入外源基因,常规培养即可实现肝细胞的扩增,所获得的肝细胞可传代;还可继续成熟培养,以获得功能性人肝成熟细胞。
Latest bibliographic data on file with the International Bureau