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1. WO2020062282 - LOW-TEMPERATURE PRESERVATION SOLUTION FOR CELLS, SODIUM ALGINATE GEL, CELL GEL, PREPARATION METHOD, USE METHOD AND APPLICATION THEREOF

Publication Number WO/2020/062282
Publication Date 02.04.2020
International Application No. PCT/CN2018/109187
International Filing Date 30.09.2018
IPC
A01N 1/02 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
A61K 9/06 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9Medicinal preparations characterised by special physical form
06Ointments; Bases therefor
A61K 35/28 2015.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
28Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
A61L 15/22 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
15Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
22containing macromolecular materials
CPC
A01N 1/02
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
A61K 35/28
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
28Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
A61K 9/06
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9Medicinal preparations characterised by special physical form
06Ointments; Bases therefor; ; Other semi-solid forms, e.g. creams, sticks, gels
A61L 15/22
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
15Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
22containing macromolecular materials
Applicants
  • 成都远山原力生物科技有限公司 CHENGDU YUANSHAN BIOTECHNOLOGY CO. LTD [CN]/[CN]
Inventors
  • 杨远 YANG, Yuan
  • 袁祥 YUAN, Xiang
  • 韩飞 HAN, Fei
  • 张中伟 ZHANG, Zhongwei
  • 潘红伟 PAN, Hongwei
Agents
  • 成都顶峰专利事务所(普通合伙) CHENGDU DINGFENG PATENT OFFICE (GENERAL PARTNER)
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) LOW-TEMPERATURE PRESERVATION SOLUTION FOR CELLS, SODIUM ALGINATE GEL, CELL GEL, PREPARATION METHOD, USE METHOD AND APPLICATION THEREOF
(FR) SOLUTION DE CONSERVATION À BASSE TEMPÉRATURE POUR CELLULES, GEL D'ALGINATE DE SODIUM, GEL CELLULAIRE, PROCÉDÉ DE PRÉPARATION, PROCÉDÉ D'UTILISATION ET SON APPLICATION
(ZH) 一种细胞低温保存液、海藻酸钠凝胶、细胞凝胶、制备方法、使用方法及其应用
Abstract
(EN)
The present invention relates to the technical field of preservation, regeneration and repair of cells, and disclosed thereby is a low-temperature preservation solution for cells, mainly prepared from the following components in percent by mass: 0.8%-1.5% of mannitol, 2%-3% of human serum albumin, 3%-7% of low-molecular dextran, 0.1-0.3% of glutathione, 0.5%-2% of epigallocatechin gallate, 3%-5% of adenosine, 0.5%-1% of glucose, 0.8% of NaCl, 0.2% of KCl, 0.1% of CaCl2 and the balance of water. Also provided by the present invention is a sodium alginate gel, comprising sodium alginate, calcium chloride, hyaluronic acid and the low-temperature preservation solution for cells, and also provided thereby are a cell gel and a preparation method therefor as well as the application of the low-temperature preservation solution for cells, the sodium alginate gel and the cell gel. The present invention has the advantages of the low-temperature preservation solution for cells being able to maintain cell activity at 2-8°C for a long duration, the sodium alginate gel being able to be used as a temporary short-term preservation material for cells from various sources and brackets for cell tissue engineering, and the cell gel may be used as a medical wet wound dressing and may be used for repairing soft tissues, refractory-healing wound skins and mucosal wound injuries.
(FR)
La présente invention concerne le domaine technique de la conservation, de la régénération et de la réparation de cellules, et concerne ainsi une solution de conservation à basse température pour cellules, qui est principalement préparée à partir des composants suivants exprimés en pourcentage en masse : 0,8 % à 1,5 % de mannitol, 2 % à 3 % de sérumalbumine humaine, 3 % à 7 % de dextrane de faible poids moléculaire, 0,1 à 0,3 % de glutathion, 0,5 % à 2 % de gallate d'épigallocatéchine, 3 % à 5 % d'adénosine, 0,5 % à 1 % de glucose, 0,8 % de NaCl, 0,2 % de KCl, 0,1 % de CaCl2 et le reste d'eau. La présente invention concerne également un gel d'alginate de sodium, comprenant de l'alginate de sodium, du chlorure de calcium, de l'acide hyaluronique et la solution de conservation à basse température pour cellules, et elle concerne également un gel cellulaire et son procédé de préparation ainsi que l'application de la solution de conservation à basse température pour cellules, du gel d'alginate de sodium et du gel cellulaire. La présente invention présente les avantages de la solution de conservation à basse température pour cellules pouvant maintenir une activité cellulaire à 2 à 8 °C pendant une longue durée, le gel d'alginate de sodium pouvant être utilisé comme substance de conservation temporaire à court terme pour des cellules provenant de diverses sources et supports pour génie tissulaire cellulaire, et le gel cellulaire pouvant être utilisé en tant que pansement médical humide pour plaies et pouvant être utilisé pour réparer des tissus mous, des peaux de plaie cicatrisantes réfractaires et des lésions des muqueuses.
(ZH)
本发明属于细胞保存和再生修复技术领域,公开了一种细胞低温保存液,主要由以下质量百分含量的组分组成:0.8%~1.5%甘露醇、2%~3%人血白蛋白、3%~7%低分子右旋糖苷、0.1~0.3%谷胱甘肽、0.5%~2%益多酚、3%~5%腺苷、0.5%~1%葡萄糖、0.8%NaCl、0.2%KCl和0.1%CaCl 2,余量为水。本发明提供了一种海藻酸钠凝胶,包括海藻酸钠、氯化钙、透明质酸和前述细胞低温保存液等,还提供了一种细胞凝胶及其制备方法,以及细胞低温保存液、海藻酸钠凝胶和细胞凝胶的应用。本发明的优点在于,细胞低温保存液可在2~8℃下较长时间保持细胞活性,海藻酸钠凝胶可作为各种来源的细胞的临时短期保存材料和细胞组织工程的支架,细胞凝胶可作为医用湿性伤口敷料,用于软组织、难愈性伤口皮肤及粘膜创面损伤的修复。
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