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1. WO2020061690 - A PASSIVE MIXING MICROFLUIDIC URINARY ALBUMIN CHIP (UAL-CHIP) FOR CHRONIC KIDNEY DISEASE ASSESSMENT

Publication Number WO/2020/061690
Publication Date 02.04.2020
International Application No. PCT/CA2019/051362
International Filing Date 24.09.2019
IPC
G01N 21/78 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
78producing a change of colour
G01N 21/64 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
CPC
B01L 2300/0867
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0861Configuration of multiple channels and/or chambers in a single devices
0867Multiple inlets and one sample wells, e.g. mixing, dilution
B01L 2300/0883
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0861Configuration of multiple channels and/or chambers in a single devices
0883Serpentine channels
B01L 2400/0457
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
04Moving fluids with specific forces or mechanical means
0403specific forces
0457passive flow or gravitation
B01L 3/502776
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502769characterised by multiphase flow arrangements
502776specially adapted for focusing or laminating flows
G01N 2021/7756
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
7756Sensor type
G01N 2021/7786
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
7769Measurement method of reaction-produced change in sensor
7786Fluorescence
Applicants
  • UNIVERSITY OF MANITOBA [CA]/[CA]
Inventors
  • LIN, Francis
  • WU, Jiandong
  • RIGATTO, Claudio
  • KOMENDA, Paul
  • TANGRI, Navdeep
  • ZHANG, Gong
Agents
  • ADE & COMPANY INC.
Priority Data
62/735,29524.09.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) A PASSIVE MIXING MICROFLUIDIC URINARY ALBUMIN CHIP (UAL-CHIP) FOR CHRONIC KIDNEY DISEASE ASSESSMENT
(FR) PUCE D'ALBUMINE URINAIRE (PUCE UAL) MICROFLUIDIQUE À MÉLANGE PASSIF PERMETTANT L'ÉVALUATION D'UNE MALADIE RÉNALE CHRONIQUE
Abstract
(EN)
Described herein is a method for mixing unequal amounts of two reagents to produce a detectable reaction in a microfluidic chip. In one example, there is a fluorescent microfluidic urinary albumin chip (UAL-Chip) that exploits the nonimmunological fluorescent assay. In this chip, we constructed a passive and continuous mixing module, in which the loading process requires only an inexpensive dropper, and the signal is stable over time, as discussed below. We applied a pressure- balancing strategy based on the immiscible oil coverage which highly improves the precision in controlling the mixing ratio of sample and dye. The UAL-Chip has achieved an estimated limit of detection (LOD) of 8.4 µg/ml using albumin standards, which is below the 30 µg albumin per ml urine level considered to be indicative of kidney damage.
(FR)
L'invention concerne une méthode de mélange de quantités inégales de deux réactifs afin de produire une réaction détectable dans une puce microfluidique. Dans un exemple, l'invention concerne une puce d'albumine urinaire (puce UAL) microfluidique fluorescente exploitant le dosage fluorescent non immunologique. Dans ladite puce, nous avons construit un module de mélange passif et continu, dans lequel le processus de chargement ne nécessite qu'un compte-gouttes peu coûteux et le signal est stable dans le temps, comme décrit ci-dessous. Nous avons appliqué une stratégie d'équilibrage de pression fondée sur la couverture d'huile non miscible qui améliore fortement la précision dans le contrôle du rapport de mélange d'échantillon et de colorant. La puce UAL a atteint une limite de détection estimée (LOD) de 8,4 µg/ml en utilisant des normes d'albumine, ce qui est inférieur à 30 µg d'albumine par ml de niveau d'urine considéré comme indiquant une lésion rénale.
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