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1. WO2020006787 - CELL LINE WITH METTL3 GENE KNOCKED OUT AND CONSTRUCTION METHOD THEREFOR, AND INTERFERENCE VECTOR

Publication Number WO/2020/006787
Publication Date 09.01.2020
International Application No. PCT/CN2018/096554
International Filing Date 21.07.2018
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
867
Retroviral vectors
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
91
Cell lines
C12N 5/10 (2006.01)
C12N 15/867 (2006.01)
C12R 1/91 (2006.01)
CPC
C12N 15/86
C12N 2510/00
C12N 2740/15043
C12N 5/0625
C12N 9/1007
Applicants
  • 浙江大学 ZHEJIANG UNIVERSITY [CN/CN]; 中国浙江省杭州市 西湖区余杭塘路866号饲料科学研究所/汪以真 WANG Yizhen/Institute of Feed Science 866 Yuhangtang Road Xihu District Hangzhou, Zhejiang 310058, CN
Inventors
  • 宗鑫 ZONG, Xin; CN
  • 汪以真 WANG, Yizhen; CN
  • 赵婧 ZHAO, Jìng; CN
Agents
  • 杭州求是专利事务所有限公司 HANGZHOU QIUSHI PATENT OFFICE CO., LTD; 中国浙江省杭州市 玉古路147号黄鸿年科技综合大楼215室林松海 LIN Songhai Room 215, Huanghongnian Science and Technology Complex Building, 147 Yugu Road Hangzhou, Zhejiang 310013, CN
Priority Data
201810733097.505.07.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) CELL LINE WITH METTL3 GENE KNOCKED OUT AND CONSTRUCTION METHOD THEREFOR, AND INTERFERENCE VECTOR
(FR) LIGNÉE CELLULAIRE DOTÉE D'UN GÈNE METTL3 INACTIVÉ ET SON PROCÉDÉ DE CONSTRUCTION, ET VECTEUR D'INTERFÉRENCE
(ZH) METTL3基因敲除的细胞系及其构建方法与干扰载体
Abstract
(EN)
Provided are a pig intestinal tract epithelium cell line with METTL3 gene knocked out and a construction method therefor. Also provided is a gene interference vector for METTL3 from a pig.
(FR)
L'invention concerne une lignée cellulaire de l'épithélium du tractus intestinal porcin dotée d'un gène METTL3 inactivé et son procédé de construction. L'invention concerne également un vecteur d'interférence génique pour le METTL3 de porcins.
(ZH)
提供了一种METTL3基因敲除的猪肠道上皮细胞系细胞系及其构建方法。还提供了一种针对猪源METTL3的基因干扰载体。
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