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1. WO2020006555 - COMPOSITIONS COMPRISING BACTERIA AND METHODS FOR CONTROLLING PLANT PESTS AND IMPROVING PLANT HEALTH

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

What is claimed is:

1. A composition comprising a cell, a spore, a forespore, or a combination of cells, spores, and/or forespores of at least one of bacterial strain AGR031898, AIP023234, AIP024552, AIP035573, AIP071234, AIP080021, AIP001237, AIP050674, AIP071546, AIP049805,

AIPO 16229, AIP081435, AIP082140, AIP000817, AIP060333, AIP070494, AIP015104,

AIP011586, AIPO 10884, AIP082287, AIP088026, AIP065969, AIP018514, AIP033041,

AIP092281, AIP081114, AIP046403, AIP022635, AIP070925, and AIP039063, or an active variant of any thereof, wherein the active variant comprises a cell, spore, forespore, or a combination of cells, spores and/or forespores having a genome within a Mash distance of about 0.015;

wherein said cell, spore, forespore, or combination of cells, spores and/or forespores or the active variant of any thereof is present at about 105 CFU/gram to about 1012 CFU/gram or at about 105 CFU/ml to about 1012 CFU/ml, and wherein an effective amount of said composition controls a plant pest or improves an agronomic trait of interest of a plant.

2. The composition of claim 1, wherein said composition comprises a cell paste or a wettable powder.

3. An isolated biologically pure culture of a bacterial strain comprising a cell, a spore, a forespore, or a combination of cells, spores and/or forespores of AIP031898, AIP023234, AIP024552, AIP035573, AIP071234, AIP080021, AIP001237, AIP050674, AIP071546,

AIP049805, AIP016229, AIP081435, AIP082140, AIP000817, AIP060333, AIP070494,

AIP015104, AIP011586, AIP010884, AIP082287, AIP088026, AIP065969, AIP018514,

AIP033041, AIP092281, AIP081114, AIP046403, AIP022635, AIP070925, and AIP039063, or an active variant of any thereof, wherein the active variant comprises a cell, spore, forespore, or combination of cells, spores and/or forespores having a genome within a Mash distance of about 0.015;

wherein an effective amount of said cell, spore, forespore, or combination of cells, spores and/or forespores controls a plant pest or improves an agronomic trait of interest of a plant.

4. The composition of claim 1 or 2, or the isolated biologically pure culture of claim 3, wherein said plant pest comprises an insect pest.

5. The composition of claim 1 or 2, or the isolated biologically pure culture of claim 3, wherein said plant pest comprises a Coleoptera insect or Hemiptera insect.

6. The composition of claim 1 or 2, or the isolated biologically pure culture of claim 3, wherein said Coleoptera insect is a corn rootworm, Colorado potato beetle, or a sweet potato weevil.

7. The composition of claim 1 or 2, or the isolated biologically pure culture of claim 3, wherein said bacterial strain or active variant thereof is resistant to a biocide selected from a herbicide, a fungicide, a pesticide, insecticide, or a crop protection chemical.

8. The composition or the isolated biologically pure culture of claim 7, wherein said bacterial strain or active variant thereof is selected under herbicide, fungicide, pesticide, insecticide, or other crop protection chemical pressure and is resistant to said herbicide, fungicide, pesticide, insecticide, or other crop protection chemical.

9. The composition or the isolated biologically pure culture of claim 7, wherein said bacterial strain or active variant has been transformed with a herbicide resistance gene rendering the cell, spore, forespore, or combination of cells, spores, and/or forespores or active variant thereof herbicide resistant.

10. A method for controlling a plant pest population comprising contacting said population with an effective amount of the composition of any one of claims 1, 2, or 4-9 or the isolated biologically pure culture of any one of claims 3-9, wherein said bacterial strain controls said plant pest.

11. A method for growing a plant susceptible to a plant pest or plant disease caused by a plant pest or improving an agronomic trait of interest in a plant comprising applying to the plant an effective amount of a cell, a spore, a forespore, or a combination of cells, spores and/or forespores of at least one of bacterial strain AIP031898, AIP023234, AIP024552, AIP035573, AIP071234, AIP080021, AIP001237, AIP050674, AIP071546, AIP049805, AIP016229, AIP081435,

AIP082140, AIP000817, AIP060333, AIP070494, AIP015104, AIP011586, AIP010884,

AIP082287, AIP088026, AIP065969, AIP018514, AIP033041, AIP092281, AIP081114,

AIP046403, AIP022635, AIP070925, and AIP039063, or an active variant of any thereof wherein

the active variant comprises a cell, spore, forespore, or a combination of cells, spores and/or forespores having a genome within a Mash distance of about 0.015;

wherein said effective amount controls said plant pest or improves the agronomic trait of interest.

12. A method of controlling a plant pest in an area of cultivation comprising:

(a) planting the area of cultivation with seeds or plants susceptible to the plant pest; and

(b) applying to the seed or plant susceptible to the plant pest an effective amount of a cell, a spore, a forespore, or a combination of cells, spores and/or forespores of at least one bacterial strain comprising AIP031898, AIP023234, AIP024552, AIP035573, AIP071234, AIP080021, AIP001237, AIP050674, AIP071546, AIP049805, AIP016229, AIP081435, AIP082140,

AIP000817, AIP060333, AIP070494, AIP015104, AIP011586, AIP010884, AIP082287,

AIP088026, AIP065969, AIP018514, AIP033041, AIP092281, AIP081114, AIP046403,

AIP022635, AIP070925, and AIP039063, or an active variant of any thereof, wherein the active variant comprises a cell, spore, forespore, or combination of cells, spores and/or forespores having a genome within a Mash distance of about 0.015; and

wherein said effective amount comprises at least about 1012 to 1016 colony forming units (CFU) per hectare.

13. A method of treating or preventing a plant disease or damage caused by a plant pest comprising applying to a plant having said plant pest, said plant disease, said damage, susceptible to said plant pest, or at risk of developing said plant disease or damage an effective amount of a cell, a spore, a forespore, or a combination of cells, spores and/or forespores of at least one of bacterial strain AIP031898, AIP023234, AIP024552, AIP035573, AIP071234, AIP080021, AIP001237, AIP050674, AIP071546, AIP049805, AIP016229, AIP081435, AIP082140,

AIP000817, AIP060333, AIP070494, AIP015104, AIP011586, AIP010884, AIP082287,

AIP088026, AIP065969, AIP018514, AIP033041, AIP092281, AIP081114, AIP046403,

AIP022635, AIP070925, and AIP039063, or an active variant of any thereof, wherein the active variant comprises a bacterial strain having a genome within a Mash distance of about 0.015;

wherein said effective amount comprises at least about 1012 to 1016 CFU per hectare, and wherein said cell, spore, forespore, or a combination of cells, spores and/or forespores controls the plant pest that causes the plant disease.

14. The method of any one of claims 11-13, where said seed or plant is a soybean.

15. The method of any one of claims 11-14, wherein said plant pest comprises an insect pest.

16. The method of claim 15, wherein said insect pest comprises a Coleoptera insect or Hemiptera insect.

17. The method of claim 16, wherein the Coleoptera insect is a corn rootworm,

Colorado potato beetle, or a sweet potato weevil.

18. The method of any one of claims 11-17, wherein said method further comprises applying an effective amount of a biocide, wherein said effective amount of the biocide selectively controls an organism of interest while not significantly damaging said seed or plant.

19. A method of making a modified bacterial strain comprising:

(a) providing a population of a cell, a spore, a forespore, or a combination of cells, spores and/or forespores of at least one bacterial strain comprising AIP031898, AIP023234, AIP024552, AIP035573, AIP071234, AIP080021, AIP001237, AIP050674, AIP071546, AIP049805,

AIPO 16229, AIP081435, AIP082140, AIP000817, AIP060333, AIP070494, AIP015104,

AIP011586, AIPO 10884, AIP082287, AIP088026, AIP065969, AIP018514, AIP033041,

AIP092281, AIP081114, AIP046403, AIP022635, AIP070925, and AIP039063, or an active variant of any thereof, wherein the active variant comprises a cell, spore, forespore, or a combination of cells, spores and/or forespores having a genome within a Mash distance of about 0.015, wherein said population is susceptible to a biocide of interest;

(b) culturing said population in the presence of a biocide of interest; and,

(c) selecting a modified bacterial strain having an increased resistance to said biocide of interest.