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1. WO2020006494 - CELL-STORED BARCODED DEEP MUTATIONAL SCANNING LIBRARIES AND USES OF THE SAME

Publication Number WO/2020/006494
Publication Date 02.01.2020
International Application No. PCT/US2019/039952
International Filing Date 28.06.2019
IPC
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005
from viruses
01
DNA viruses
03
Herpetoviridae, e.g. pseudorabies virus
05
Epstein-Barr virus
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005
from viruses
08
RNA viruses
15
Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
155
Lentiviridae, e.g. human immunodeficiency virus (HIV), visna-maedi virus, equine infectious anaemia virus
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
20
Methods specially adapted for identifying library members
04
Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40
Libraries per se, e.g. arrays, mixtures
02
Libraries contained in or displayed by micro-organisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only micro-organisms or vectors
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40
Libraries per se, e.g. arrays, mixtures
04
Libraries containing only organic compounds
06
Libraries containing nucleotides or polynucleotides, or derivatives thereof
08
Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50
Methods of creating libraries, e.g. combinatorial synthesis
08
Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
10
involving encoding steps
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
70
Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
C07K 14/005 (2006.01)
C07K 14/155 (2006.01)
C12N 15/10 (2006.01)
C40B 20/04 (2006.01)
C40B 40/02 (2006.01)
C40B 40/08 (2006.01)
CPC
C07K 14/005
C07K 14/155
C12N 15/10
C12N 15/1034
C12N 15/1093
C40B 20/04
Applicants
  • FRED HUTCHINSON CANCER RESEARCH CENTER [US/US]; 1100 Fairview Avenue North Seattle, Washington 98109, US
  • UNIVERSITY OF WASHINGTON [US/US]; 4545 ROOSEVELT WAY NE SUITE 400 Seattle, Washington 98105, US
Inventors
  • BLOOM, Jesse; US
  • DINGENS, Adam S.; US
  • DUSENBURY, Katharine; US
  • RADFORD, Caelan; US
Agents
  • WINGER, C. Rachal; US
  • NGUYEN, Thu; US
Priority Data
62/692,39829.06.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CELL-STORED BARCODED DEEP MUTATIONAL SCANNING LIBRARIES AND USES OF THE SAME
(FR) BIBLIOTHÈQUES DE BALAYAGE DE MUTATION PROFOND À CODE À BARRES STOCKÉES DANS UNE CELLULE, ET UTILISATIONS DESDITES BIBLIOTHÈQUES
Abstract
(EN)
Cell-stored barcoded viral protein deep mutational scanning libraries are described. The libraries can be used to map resistance mutations to therapeutic treatments. The libraries can be used to predict viruses that become resistant to therapeutic compounds and/or may more easily evolve to infect new species. The libraries can also be used to more safely study dangerous viruses that normally require high safety biocontainment facilities. The libraries include features that allow efficient collection and assessment of informative data, obviating many bottlenecks of previous approaches.
(FR)
L'invention concerne des bibliothèques de balayage mutationnel profond de protéines virales à code à barres stockées dans une cellule. Les bibliothèques peuvent être utilisées pour apparier des mutations de résistance avec des traitements thérapeutiques. Les bibliothèques peuvent être utilisées pour prédire des virus qui deviennent résistants à des composés thérapeutiques et/ou peuvent évoluer plus facilement pour infecter de nouvelles espèces. Les bibliothèques peuvent également être utilisées pour étudier plus sûrement les virus dangereux qui nécessitent normalement des installations de confinement biologique de haute sécurité. Les bibliothèques comprennent des caractéristiques qui permettent une collecte et une évaluation efficaces de données informatives, ce qui permet d'éviter les nombreux goulots d'étranglement des approches précédentes.
Latest bibliographic data on file with the International Bureau