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1. WO2020005980 - RNA-GUIDED EFFECTOR PROTEINS AND METHODS OF USE THEREOF

Publication Number WO/2020/005980
Publication Date 02.01.2020
International Application No. PCT/US2019/039041
International Filing Date 25.06.2019
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
22
Ribonucleases
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C12N 9/22 (2006.01)
C12N 15/00 (2006.01)
CPC
C12N 9/22
Applicants
  • THE REGENTS OF THE UNIVERSITY OF CALIFORNIA [US/US]; 1111 Franklin Street 12th Floor Oakland, California 94607, US
Inventors
  • DOUDNA, Jennifer A.; US
  • SAVAGE, David Frank; US
  • HIGGINS, Sean A.; US
  • OAKES, Benjamin L.; US
Agents
  • BORDEN, Paula A.; US
Priority Data
62/690,28226.06.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) RNA-GUIDED EFFECTOR PROTEINS AND METHODS OF USE THEREOF
(FR) PROTÉINES EFFECTRICES GUIDÉES PAR ARN ET LEURS PROCÉDÉS D'UTILISATION
Abstract
(EN)
The present disclosure provides an RNA-guided effector polypeptide having a length that is less than Streptococcus pyogenes Cas9, and that retains the ability, when complexed with a guide RNA, to bind to a target nucleic acid. The present disclosure provides a fusion polypeptide comprising: i) an RNA-guided effector polypeptide of the present disclosure; and ii) a fusion partner. The present disclosure further provides nucleic acids encoding an RNA-guided effector polypeptide, or a fusion polypeptide, of the present disclosure. Methods of using an RNA-guided effector polypeptide, or a fusion polypeptide, of the present disclosure are also provided.
(FR)
La présente invention concerne un polypeptide effecteur guidé par ARN ayant une longueur qui est inférieure à celle de Cas9 de Streptococcus pyogenes, et qui conserve la capacité, lorsqu'il est complexé avec un ARN guide, de se lier à un acide nucléique cible. La présente invention concerne un polypeptide de fusion comprenant : i) un polypeptide effecteur guidé par ARN selon la présente invention; et ii) un partenaire de fusion. La présente invention concerne en outre des acides nucléiques codant pour un polypeptide effecteur guidé par ARN, ou un polypeptide de fusion, selon la présente invention. L'invention concerne également des procédés d'utilisation d'un polypeptide effecteur guidé par ARN, ou d'un polypeptide de fusion, selon la présente invention.
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