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1. WO2020004571 - ADDITIVE FOR CULTURING STEM CELLS, CULTURING MEDIUM, AND CULTURING METHOD

Publication Number WO/2020/004571
Publication Date 02.01.2020
International Application No. PCT/JP2019/025666
International Filing Date 27.06.2019
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
073
Embryonic cells or tissues; Foetal cells or tissues
0735
Embryonic stem cells; Embryonic germ cells
C12N 5/10 (2006.01)
C12N 5/0735 (2010.01)
CPC
C12N 5/10
Applicants
  • 味の素株式会社 AJINOMOTO CO., INC. [JP/JP]; 東京都中央区京橋一丁目15番1号 15-1, Kyobashi 1-chome, Chuo-ku, Tokyo 1048315, JP
Inventors
  • 伊藤 健一郎 ITO, Kenichiro; JP
Agents
  • 高島 一 TAKASHIMA, Hajime; JP
Priority Data
2018-12253227.06.2018JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) ADDITIVE FOR CULTURING STEM CELLS, CULTURING MEDIUM, AND CULTURING METHOD
(FR) ADDITIF POUR CULTURE DE CELLULES SOUCHES, MILIEU DE CULTURE, ET MÉTHODE DE CULTURE
(JA) 幹細胞の培養用添加物および培養用培地、ならびに培養方法
Abstract
(EN)
The present invention is characterized in that it provides: an additive for culturing stem cells, comprising a polysaccharide other than dextran sulfate having a molecular weight of 4,000 kDa-40,000 kDa; and a medium for culturing stem cells, comprising a polysaccharide other than dextran sulfate having a molecular weight of 4,000 kDa-40,000 kDa. The present invention is also characterized by carrying out suspension culture of stem cells using a medium for culturing stem cells, comprising a polysaccharide other than dextran sulfate having a molecular weight of 4,000 kDa-40,000 kDa. The present invention makes it possible to improve the rate of cell mass formation of stem cells, to control the morphology thereof, and also to improve the rate at which stem cells multiply and the rate that stem cells remain undifferentiated, during suspension culture of stem cells.
(FR)
La présente invention est caractérisée en ce qu'elle comprend : un additif pour la culture de cellules souches, comprenant un polysaccharide autre que le sulfate de dextrane ayant un poids moléculaire de 4 000 kDa à 40 000 kDa ; et un milieu pour la culture de cellules souches, comprenant un polysaccharide autre que le sulfate de dextrane ayant un poids moléculaire de 4 000 kDa à 40 000 kDa. La présente invention est également caractérisée par la réalisation d'une culture en suspension de cellules souches à l'aide d'un milieu pour la culture de cellules souches, comprenant un polysaccharide autre que le sulfate de dextrane ayant un poids moléculaire de 4 000 kDa à 40 000 kDa. La présente invention permet d'améliorer la vitesse de formation d'une masse cellulaire de cellules souches, de contrôler leur morphologie, et également d'améliorer la vitesse à laquelle les cellules souches se multiplient et la vitesse à laquelle les cellules souches restent indifférenciées, pendant la culture en suspension de cellules souches.
(JA)
本発明は、分子量が4,000kDa~40,000kDaであるデキストラン硫酸以外の多糖類を含有する幹細胞の培養用添加物、または分子量が4,000kDa~40,000kDaであるデキストラン硫酸以外の多糖類を含有する幹細胞の培養用培地とし、分子量が4,000kDa~40,000kDaであるデキストラン硫酸以外の多糖類を含有する幹細胞の培養用培地にて、幹細胞の浮遊培養を行うことを特徴とする。 本発明によれば、幹細胞の浮遊培養において、幹細胞の細胞塊形成率を向上させ、かつその形状を制御し、さらに幹細胞の増殖率および未分化維持率を向上させることができる。
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