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1. WO2020004473 - METHOD FOR PRODUCING CELLULASE AGENT, AND METHOD FOR PRODUCING SACCHARIFICATION/FERMENTATION PRODUCT IN WHICH SAID CELLULASE AGENT IS USED

Publication Number WO/2020/004473
Publication Date 02.01.2020
International Application No. PCT/JP2019/025404
International Filing Date 26.06.2019
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
42
acting on beta-1, 4-glucosidic bonds, e.g. cellulase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
02
containing a hydroxy group
04
acyclic
06
Ethanol, i.e. non-beverage
C12N 9/42 (2006.01)
C12N 1/16 (2006.01)
C12P 7/06 (2006.01)
CPC
C12N 1/16
C12P 7/06
Applicants
  • 関西化学機械製作株式会社 KANSAI CHEMICAL ENGINEERING CO., LTD. [JP/JP]; 兵庫県尼崎市南七松町2丁目9番7号 9-7, Minaminanamatsucho 2-chome, Amagasaki-shi, Hyogo 6600053, JP
  • Bio-energy株式会社 BIO-ENERGY CORPORATION [JP/JP]; 兵庫県尼崎市南七松町2丁目9番7号 9-7, Minaminanamatsucho 2-chome, Amagasaki-shi, Hyogo 6600053, JP
Inventors
  • ▲濱▼ 真司 HAMA, Shinji; JP
  • 木原 真希 KIHARA, Maki; JP
  • 野田 秀夫 NODA, Hideo; JP
Agents
  • 中道 佳博 NAKAMICHI, Yoshihiro; JP
  • 藤原 有希 FUJIWARA, Yuki; JP
Priority Data
2018-12299728.06.2018JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD FOR PRODUCING CELLULASE AGENT, AND METHOD FOR PRODUCING SACCHARIFICATION/FERMENTATION PRODUCT IN WHICH SAID CELLULASE AGENT IS USED
(FR) MÉTHODE DE PRODUCTION D'UN AGENT CELLULASE ET MÉTHODE DE PRODUCTION D'UN PRODUIT DE SACCHARIFICATION/FERMENTATION DANS LEQUEL LEDIT AGENT CELLULASE EST UTILISÉ
(JA) セルラーゼ剤の製造方法ならびに当該セルラーゼ剤を用いた糖化発酵産物の製造方法
Abstract
(EN)
Disclosed is a method for producing a cellulase agent. This method includes a step for culturing microorganisms of the genus Trichoderma at a pH of at least 1 and less than 5 in a medium containing a polysaccharide that has at least two β-1,4-bonded glucoses to produce a cellulase agent, the ratio of the β-glucosidase activity in the cellulase agent to the filter paper decomposition activity of the cellulase agent being lower than 3. Also disclosed are a method for producing a saccharification/fermentation product and a method for culturing surface layer presentation microorganisms using a culture medium that contains a cellulose material treated by a cellulase agent produced by the above method. These methods make it possible to produce a saccharification/fermentation product efficiently from a cellulose-based biomass irrespective of the location environment and production scale of a manufacturer.
(FR)
L'invention porte sur une méthode de production d'un agent cellulase. Cette méthode comprend une étape de culture de micro-organismes du genre Trichoderma à un pH d'au moins 1 et inférieur à 5 dans un milieu contenant un polysaccharide qui a au moins deux glucoses à liaison β-1,4 pour produire un agent cellulase, le rapport de l'activité β-glucosidase dans l'agent cellulase à l'activité de décomposition du papier filtre de l'agent cellulase étant inférieur à 3. L'invention concerne également une méthode de production d'un produit de saccharification/fermentation et une méthode de culture de micro-organismes de présentation de couche de surface à l'aide d'un milieu de culture qui contient un matériau cellulosique traité par un agent cellulase produit par la méthode ci-dessus. Ces méthodes permettent de produire efficacement un produit de saccharification/fermentation à partir d'une biomasse à base de cellulose indépendamment de l'environnement de localisation et de l'échelle de production d'un fabricant.
(JA)
セルラーゼ剤を製造する方法を開示する。この方法は、トリコデルマ属微生物を、pH1以上かつ5未満にて、少なくとも2つのグルコースがβ-1,4結合した多糖を含有する培地にて培養し、セルラーゼ剤を生産させる工程を含み、該セルラーゼ剤の濾紙分解活性に対する該セルラーゼ剤におけるβ-グルコシダーゼ活性の比率が3より低い。さらに、上記方法で製造されたセルラーゼ剤で処理したセルロース材料を含有する培地を用いる、糖化発酵産物の製造方法および表層提示微生物の培養方法を開示する。これらの方法によれば、製造者の立地環境や生産規模に関わらず、セルロース系バイオマスから糖化発酵産物を効率よく製造し得る。
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