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1. WO2020004470 - METHOD FOR EVALUATING CYTOTOXICITY OF GAS

Publication Number WO/2020/004470
Publication Date 02.01.2020
International Application No. PCT/JP2019/025400
International Filing Date 26.06.2019
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
C12Q 1/02 (2006.01)
G01N 33/50 (2006.01)
CPC
C12Q 1/02
G01N 33/00
G01N 33/50
Applicants
  • ダイキン工業株式会社 DAIKIN INDUSTRIES, LTD. [JP/JP]; 大阪府大阪市北区中崎西2丁目4番12号 梅田センタービル Umeda Center Building, 4-12, Nakazaki-Nishi 2-Chome, Kita-ku, Osaka-shi, Osaka 5308323, JP
  • 国立大学法人 東京大学 THE UNIVERSITY OF TOKYO [JP/JP]; 東京都文京区本郷七丁目3番1号 3-1, Hongo 7-chome, Bunkyo-ku, Tokyo 1138654, JP
Inventors
  • 池嶋 育美 IKEJIMA, Ikumi; JP
  • 松本 博士 MATSUMOTO, Hiroshi; JP
  • 澤田 光平 SAWADA, Kohei; JP
Agents
  • 特許業務法人三枝国際特許事務所 SAEGUSA & PARTNERS; 大阪府大阪市中央区道修町1-7-1 北浜TNKビル Kitahama TNK Building, 1-7-1, Doshomachi, Chuo-ku, Osaka-shi, Osaka 5410045, JP
Priority Data
2018-12287628.06.2018JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD FOR EVALUATING CYTOTOXICITY OF GAS
(FR) PROCÉDÉ PERMETTANT L'ÉVALUATION DE LA CYTOTOXICITÉ D'UN GAZ
(JA) 気体の細胞毒性評価方法
Abstract
(EN)
To provide a method for evaluating cytotoxicity of a gas, the method being applicable even to cells present in a cell culture solution, i.e., applicable even to cells other than bronchial or lung-derived cells. A method for evaluating toxicity of a gas to cells, characterized by having, in order, a gas dissolution step for dissolving a gas in a cell culture solution, and a cell evaluation step for measuring a cell function.
(FR)
L'invention vise à fournir un procédé pour évaluer la cytotoxicité d'un gaz, le procédé pouvant s'appliquer même à des cellules présentes dans une solution de culture cellulaire, c'est-à-dire pouvant s'appliquer même à des cellules autres que des cellules bronchiques ou d'origine pulmonaire. L'invention concerne à cet effet un procédé pour évaluer la toxicité d'un gaz vis-à-vis de cellules, caractérisé en ce qu'il comprend, dans l'ordre, une étape de dissolution du gaz pour dissoudre le gaz dans une solution de culture cellulaire, et une étape d'évaluation des cellules pour mesurer une fonction cellulaire.
(JA)
細胞培養液中に存在する細胞に対しても適用可能な、つまり、気管支や肺由来の細胞以外の細胞に対しても適用可能な、気体の細胞毒性評価方法を提供すること。 気体を細胞培養液に溶解させる気体溶解工程、 及び、細胞機能を測定する細胞評価工程をこの順に有することを特徴とする、気体による細胞への毒性評価方法。
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