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1. WO2020003833 - CELL CULTURE METHOD, METHOD FOR PRODUCING PRODUCT, AND CELL CULTURE DEVICE

Publication Number WO/2020/003833
Publication Date 02.01.2020
International Application No. PCT/JP2019/020717
International Filing Date 24.05.2019
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02
Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
04
with gas introduction means
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3
Tissue, human, animal or plant cell, or virus culture apparatus
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
C12N 5/02 (2006.01)
C12M 1/04 (2006.01)
C12M 3/00 (2006.01)
C12N 5/071 (2010.01)
CPC
C12M 1/04
C12M 3/00
Applicants
  • 富士フイルム株式会社 FUJIFILM CORPORATION [JP/JP]; 東京都港区西麻布2丁目26番30号 26-30, Nishiazabu 2-chome, Minato-ku, Tokyo 1068620, JP
Inventors
  • 中居 真一 NAKAI, Shinichi; JP
  • 谷口 幸助 TANIGUCHI, Kosuke; JP
  • 原口 暢之 HARAGUCHI, Nobuyuki; JP
  • 石原 司 ISHIHARA, Tsukasa; JP
Agents
  • 特許業務法人太陽国際特許事務所 TAIYO, NAKAJIMA & KATO; 東京都新宿区新宿4丁目3番17号 3-17, Shinjuku 4-chome, Shinjuku-ku, Tokyo 1600022, JP
Priority Data
2018-12232227.06.2018JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) CELL CULTURE METHOD, METHOD FOR PRODUCING PRODUCT, AND CELL CULTURE DEVICE
(FR) PROCÉDÉ DE CULTURE CELLULAIRE, PROCÉDÉ POUR LA PRODUCTION D'UN PRODUIT ET DISPOSITIF DE CULTURE CELLULAIRE
(JA) 細胞培養方法、生産物の製造方法、及び細胞培養装置
Abstract
(EN)
To provide: a cell culture method which comprises using a culture container housing a cell suspension containing cells, and culturing the cells by releasing a gas containing 30 vol% or more of oxygen to the cell suspension from a sparger disposed in the culture container, wherein the average pore size of a gas releasing part of the sparger is 1-300 μm inclusive, and the surface area (A m2) of the gas releasing part of the sparger, the amount (X m3) of the cell suspension in the culture container and the flow rate (Q m3/min) of the gas released from the sparger satisfy a specific relationship; a method for producing a product, said method comprising the cell culture method; and a cell culture device which makes it possible to carry out the cell culture method.
(FR)
L'invention concerne : un procédé de culture cellulaire, qui comprend l'utilisation d'un récipient de culture contenant une suspension de cellules contenant des cellules et la culture des cellules par libération d'un gaz contenant 30 % en volume ou plus d'oxygène dans la suspension de cellules à partir d'un diffuseur disposé dans le récipient de culture, la taille moyenne des pores d'une partie de libération de gaz du diffuseur étant de 1 à 300 µm, bornes incluses, et la surface (A en m2) de la partie de libération de gaz du diffuseur, la quantité (X en m3) de la suspension de cellules dans le récipient de culture et le débit (Q en m3/min) du gaz libéré par le diffuseur satisfaisant à une relation particulière ; un procédé pour la production d'un produit, ledit procédé comprenant le procédé de culture cellulaire ; et un dispositif de culture cellulaire qui permet de mettre en œuvre le procédé de culture cellulaire.
(JA)
細胞を含む細胞懸濁液を収容する培養容器を用い、上記培養容器内に配置されたスパージャーから酸素を30体積%以上含むガスを上記細胞懸濁液に放出して細胞を培養することを含み、上記スパージャーにおけるガス放出部の平均孔径が1μm以上300μm以下であり、上記スパージャーにおけるガス放出部の表面積A mと、上記培養容器内の上記細胞懸濁液の量X mと、上記スパージャーから放出される上記ガスの流量Q m/minとが、特定の関係を満たす細胞培養方法、上記細胞培養方法を含む生産物の製造方法、及び、上記細胞培養方法を可能とする細胞培養装置を提供すること。
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