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1. (WO2020003752) PROTEIN HAVING FRUCTOSYL VALYL HISTIDINE OXIDASE ACTIVITY
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2020/003752 International Application No.: PCT/JP2019/018491
Publication Date: 02.01.2020 International Filing Date: 09.05.2019
IPC:
C12N 15/53 (2006.01) ,C12M 1/34 (2006.01) ,C12N 1/15 (2006.01) ,C12N 1/19 (2006.01) ,C12N 1/21 (2006.01) ,C12N 5/10 (2006.01) ,C12N 9/04 (2006.01) ,C12N 15/63 (2006.01) ,C12Q 1/26 (2006.01)
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
53
Oxidoreductases (1)
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
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Apparatus for enzymology or microbiology
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Measuring or testing with condition measuring or sensing means, e.g. colony counters
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
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Fungi; Culture media therefor
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modified by introduction of foreign genetic material
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
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Fungi; Culture media therefor
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Yeasts; Culture media therefor
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modified by introduction of foreign genetic material
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
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Bacteria; Culture media therefor
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modified by introduction of foreign genetic material
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Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
04
acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
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Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
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involving oxidoreductase
Applicants:
東洋紡株式会社 TOYOBO CO., LTD. [JP/JP]; 大阪府大阪市北区堂島浜二丁目2番8号 2-8, Dojima Hama 2-chome, Kita-ku, Osaka-shi, Osaka 5308230, JP
Inventors:
川井 淳 KAWAI, Atsushi; JP
Priority Data:
2018-12184427.06.2018JP
Title (EN) PROTEIN HAVING FRUCTOSYL VALYL HISTIDINE OXIDASE ACTIVITY
(FR) PROTÉINE AYANT UNE ACTIVITÉ FRUCTOSYL VALYL HISTIDINE OXYDASE
(JA) フルクトシルバリルヒスチジンオキシダーゼ活性を有するタンパク質
Abstract:
(EN) Provided are a fructosyl amino acid oxidase having improved stability in the presence of quaternary ammonium salts, and a glycated protein measurement method utilizing the same. The present invention provides a protein having fructosyl valyl histidine oxidase activity according to any of (a) to (c): (a) a protein comprising the amino acid sequence represented by SEQ ID NO: 1, wherein glutamic acid at at least one site selected from the group consisting of the 101st, 131st, 169th, 245th, and 360th positions is substituted with another amino acid; (b) a protein comprising amino acid sequence (I), wherein one or several amino acids are deleted, substituted, or added at sites other than the 101st, 131st, 169th, 245th, and 360th positions; and (c) a protein comprising an amino acid sequence having at least 85.0% identity to the amino acid sequence represented by SEQ ID NO: 1, wherein glutamic acid corresponding to at least one site selected from the group consisting of the 101st, 131st, 169th, 245th, and 360th positions in SEQ ID NO: 1 is substituted with another amino acid.
(FR) L'invention concerne une fructosyl-acide aminé oxydase ayant une stabilité améliorée pour un sel d'ammonium quaternaire et un procédé de mesure de protéine glyquée l'utilisant. La présente invention concerne une protéine ayant une activité fructosyl valyl histidine oxydase selon l'un quelconque de (a) à (c) ci-dessous : (a) une protéine comprenant, dans la séquence d'acides aminés présentée dans SEQ ID NO : 1, une séquence d'acides aminés dans laquelle l'acide glutamique au niveau d'au moins un site sélectionné dans un groupe constitué des 101ème, 131ème, 169ème, 245ème, et 360ème positions est substitué par un autre acide aminé ; (b) une protéine comprenant, dans la séquence d'acides aminés (I), une séquence d'acides aminés dans laquelle un ou plusieurs acides aminés sont supprimés, substitués ou ajoutés au niveau de sites autres que les 101ème, 131ème, 169ème, 245ème, et 360ème positions ; et (c) une protéine comprenant, dans la séquence d'acides aminés ayant au moins 85,0 % d'identité avec la séquence d'acides aminés représentée dans SEQ ID NO : 1, une séquence d'acides aminés dans laquelle l'acide glutamique correspondant à au moins un site sélectionné dans un groupe constitué des 101ème, 131ème, 169ème, 245ème, et 360ème positions dans SEQ ID NO : 1 est substitué par un autre acide aminé.
(JA) 第四級アンモニウム塩に対する安定性が向上したフルクトシルアミノ酸オキシダーゼおよびそれを利用した糖化タンパク質の測定方法を提供する。 以下の(a)~(c)のいずれかに記載の、フルクトシルバリルヒスチジンオキシダーゼ活性を有するタンパク質。 (a)配列番号1に示されるアミノ酸配列において、101番目、131番目、169番目、245番目および360番目からなる群から選択される少なくともいずれか1箇所の部位におけるグルタミン酸が他のアミノ酸に置換されたアミノ酸配列からなるタンパク質 (b)アミノ酸配列(I)において、101番目、131番目、169番目、245番目および360番目以外の部位において、1もしくは数個のアミノ酸が欠失、置換、もしくは付加されたアミノ酸配列からなるタンパク質 (c)配列番号1に示されるアミノ酸配列と85.0%以上の同一性を有するアミノ酸配列において、配列番号1の101番目、131番目、169番目、245番目および360番目からなる群から選択される少なくともいずれか1箇所の部位に相当するグルタミン酸が他のアミノ酸に置換されたアミノ酸配列からなるタンパク質
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)