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1. WO2020002598 - BIOREACTOR FOR RNA IN VITRO TRANSCRIPTION

Publication Number WO/2020/002598
Publication Date 02.01.2020
International Application No. PCT/EP2019/067323
International Filing Date 28.06.2019
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6865 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
6865Promoter-based amplification, e.g. nucleic acid sequence-based amplification , self-sustained sequence replication or transcription-based amplification system
CPC
B01F 13/0818
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
13Other mixers; Mixing plant, including combinations of ; mixers, e.g. of; dissimilar mixers
08Magnetic mixers ; ; Mixers having magnetically driven stirrers
0818using independent floating stirring elements
B01F 15/00896
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
15Accessories for mixers ; ; Auxiliary operations or auxiliary devices; Parts or details of general application
00824Receptacles
00876Configuration of the interior
00883provided with baffles, plates or bars on the wall or the bottom
00896with vertical baffles mounted on the walls
C12N 15/1013
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
1013by using magnetic beads
C12Q 1/6865
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
C12Q 2521/119
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2521Reaction characterised by the enzymatic activity
10Nucleotidyl transfering
119RNA polymerase
C12Q 2563/143
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2563Nucleic acid detection characterized by the use of physical, structural and functional properties
143Magnetism, e.g. magnetic label
Applicants
  • CUREVAC AG [DE]/[DE]
  • TESLA GROHMANN AUTOMATION GMBH [DE]/[DE]
Inventors
  • YAZDAN PANAH, Benyamin
  • ROOS, Tilmann
  • KUNZE, Martin
  • BERTSCH, Felix
  • WOCHNER, Aniela
  • RAUEN, Michael
  • HOFFMANN, Philipp
Agents
  • MAIWALD PATENTANWALTS- UND RECHTSANWALTSGESELLSCHAFT MBH
Priority Data
PCT/EP2018/06750428.06.2018EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BIOREACTOR FOR RNA IN VITRO TRANSCRIPTION
(FR) BIORÉACTEUR POUR TRANSCRIPTION IN VITRO D'ARN
Abstract
(EN)
The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated apparatus for RNA manufacturing. Further, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention.The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP-compliant conditions. In particular, said RNA in vitro transcription reactor allows repetitive use of DNA template for various RNA in vitro transcription reactions. Further, the invention relates to an apparatus for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcribing DNA into RNA comprising said RNA in vitro transcription reactor, and, optionally, (c) a module for RNA formulation.
(FR)
La présente invention concerne un bioréacteur pour la transcription in vitro d'ARN, un procédé pour la transcription in vitro d'ARN, un module pour transcrire l'ADN en ARN, et un appareil automatisé pour la fabrication d'ARN. En outre, la présente invention a trait à l'utilisation d'un bioréacteur pour la transcription in vitro d'ARN, et concerne un réacteur de transcription in vitro d'ARN conçu pour fonctionner de manière automatisée dans des conditions conformes aux bonnes pratiques de fabrication. En particulier, ledit réacteur de transcription in vitro d'ARN permet l'utilisation répétitive d'un modèle d'ADN pour diverses réactions de transcription in vitro d'ARN. En outre, l'invention concerne un appareil pour la fabrication d'ARN comprenant (a) un module pour la synthèse d'ADN de matrice, (b) un module pour transcrire l'ADN en ARN comprenant ledit réacteur de transcription in vitro d'ARN et, éventuellement, (c) un module pour la formulation d'ARN.
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