WO2020002598 BIOREACTOR FOR RNA IN VITRO TRANSCRIPTION

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Publication Number WO/2020/002598

Publication Date 02.01.2020

International Application No. PCT/EP2019/067323

International Filing Date 28.06.2019

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CPC

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Applicants

CUREVAC AG [DE]/[DE]
TESLA GROHMANN AUTOMATION GMBH [DE]/[DE]

Inventors

YAZDAN PANAH, Benyamin
ROOS, Tilmann
KUNZE, Martin
BERTSCH, Felix
WOCHNER, Aniela
RAUEN, Michael
HOFFMANN, Philipp

Agents

MAIWALD PATENTANWALTS- UND RECHTSANWALTSGESELLSCHAFT MBH

Priority Data

PCT/EP2018/067504

28.06.2018

Publication Language English (en)

Filing Language English (EN)

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Title

(EN) BIOREACTOR FOR RNA IN VITRO TRANSCRIPTION

(FR) BIORÉACTEUR POUR TRANSCRIPTION IN VITRO D'ARN

Abstract

(EN) The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated apparatus for RNA manufacturing. Further, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention.The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP-compliant conditions. In particular, said RNA in vitro transcription reactor allows repetitive use of DNA template for various RNA in vitro transcription reactions. Further, the invention relates to an apparatus for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcribing DNA into RNA comprising said RNA in vitro transcription reactor, and, optionally, (c) a module for RNA formulation.

(FR) La présente invention concerne un bioréacteur pour la transcription in vitro d'ARN, un procédé pour la transcription in vitro d'ARN, un module pour transcrire l'ADN en ARN, et un appareil automatisé pour la fabrication d'ARN. En outre, la présente invention a trait à l'utilisation d'un bioréacteur pour la transcription in vitro d'ARN, et concerne un réacteur de transcription in vitro d'ARN conçu pour fonctionner de manière automatisée dans des conditions conformes aux bonnes pratiques de fabrication. En particulier, ledit réacteur de transcription in vitro d'ARN permet l'utilisation répétitive d'un modèle d'ADN pour diverses réactions de transcription in vitro d'ARN. En outre, l'invention concerne un appareil pour la fabrication d'ARN comprenant (a) un module pour la synthèse d'ADN de matrice, (b) un module pour transcrire l'ADN en ARN comprenant ledit réacteur de transcription in vitro d'ARN et, éventuellement, (c) un module pour la formulation d'ARN.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
10	Processes for the isolation, preparation or purification of DNA or RNA

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68	involving nucleic acids
6844	Nucleic acid amplification reactions
6865	Promoter-based amplification, e.g. nucleic acid sequence-based amplification , self-sustained sequence replication or transcription-based amplification system

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
10	Processes for the isolation, preparation or purification of DNA or RNA

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68	involving nucleic acids
6844	Nucleic acid amplification reactions
6865	Promoter-based amplification, e.g. nucleic acid sequence-based amplification , self-sustained sequence replication or transcription-based amplification system

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
21	Bioreactors or fermenters specially adapted for specific uses
18	Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes

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1. WO2020002598 - BIOREACTOR FOR RNA IN VITRO TRANSCRIPTION

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19	Preparation of compounds containing saccharide radicals
26	Preparation of nitrogen-containing carbohydrates
28	N-glycosides
30	Nucleotides
34	Polynucleotides, e.g. nucleic acids, oligoribonucleotides