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1. WO2020000465 - METHOD FOR PREPARING LIGHTR GENE KNOCKOUT MICE

Publication Number WO/2020/000465
Publication Date 02.01.2020
International Application No. PCT/CN2018/093875
International Filing Date 29.06.2018
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
64
General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
C12N 15/11 (2006.01)
C12N 15/63 (2006.01)
C12N 15/64 (2006.01)
CPC
C12N 15/11
C12N 15/63
C12N 15/64
Applicants
  • 深圳市博奥康生物科技有限公司 SHENZHEN BIOCAN TECHNOLOGIES CO., LTD. [CN/CN]; 中国广东省深圳市 南山区西丽街道珠光路134号珠光创新科技园1栋509室 毛吉炎 MAO, Jiyan Room 509, Building 1, Zhuguang Innovation Park No.134 Zhuguang Road, Xili Street, Nanshan District Shenzhen, Guangdong 518055, CN
Inventors
  • 毛吉炎 MAO, Jiyan; CN
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR PREPARING LIGHTR GENE KNOCKOUT MICE
(FR) PROCÉDÉ DE PRÉPARATION DE SOURIS À INVALIDATION DU GÈNE LIGHTR
(ZH) 一种制备LIGHTR基因敲除小鼠的方法
Abstract
(EN)
Provided is the use of the CRISPR-Cas9 system to edit the LIGHTR gene, and LIGHTR knockout mice are obtained by means of somatic cell nuclear transfer technology. sgRNA is designed for the first time in the CDS region of the mouse LIGHTR gene, and the above is cut using the CRISPR-Cas9 system, thus achieving knockout of the LIGHTR gene and obtaining a corresponding knockout mouse individual; a practical method for studying the mouse LIGHTR gene is thus provided.
(FR)
L'invention concerne l'utilisation du système CRISPR-Cas9 pour modifier le gène LIGHTR, et les souris à invalidation du gène LIGHTR qui sont obtenues au moyen d'une technologie de transfert nucléaire de cellules somatiques. L'ARNsg est conçu pour la première fois dans la région CDS du gène LIGHTR de souris, et celui-ci est coupé à l'aide du système CRISPR-Cas9, ce qui permet d'obtenir une invalidation du gène LIGHTR et d'obtenir un individu de souris à invalidation du gène correspondant ; un procédé pratique d'étude du gène LIGHTR de souris est ainsi fourni.
(ZH)
提供了利用CRISPR-Cas9系统对LIGHTR基因进行编辑,并通过体细胞核移植技术获得LIGHTR基因敲除小鼠。首次针对小鼠LIGHTR基因的CDS区设计sgRNA,并利用CRISPR-Cas9系统对其进行切割,实现了LIGHTR基因的敲除,并且获得了相应的敲除小鼠个体,为研究小鼠LIGHTR基因提供了一种切实可行的方法。
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