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1. (WO2020000462) METHOD FOR PREPARING MCDR2 GENE KNOCK-OUT MOUSE
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2020/000462 International Application No.: PCT/CN2018/093872
Publication Date: 02.01.2020 International Filing Date: 29.06.2018
IPC:
C12N 15/113 (2010.01) ,C12N 15/85 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
113
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
Applicants:
深圳市博奥康生物科技有限公司 SHENZHEN BIOCAN TECHNOLOGIES CO., LTD. [CN/CN]; 中国广东省深圳市 南山区西丽街道珠光路134号珠光创新科技园1栋509室 毛吉炎 MAO Jiyan Room 509, Building 1, Zhuguang Innovation Park No.134 Zhuguang Road, Xilii Street, Nanshan District Shenzhen, Guangdong 518055, CN
Inventors:
毛吉炎 MAO, Jiyan; CN
Priority Data:
Title (EN) METHOD FOR PREPARING MCDR2 GENE KNOCK-OUT MOUSE
(FR) PROCÉDÉ DE PRÉPARATION D'UNE SOURIS KNOCK-OUT DU GÈNE MCDR2
(ZH) 一种制备MCDR2基因敲除小鼠的方法
Abstract:
(EN) Provided is a MCDR2 gene knock-out mouse obtained by using a CRISPR-Cas9 system to edit an MCDR2 gene and performing somatic cell nuclear transfer. sgRNA is designed for the CDS region of the mouse MCDR2 gene and is cleaved by means of the CRISPR-Cas9 system to achieve knock-out of the MCDR2 gene, and then corresponding knock-out mouse individuals are obtained, a practical method for the study of the mouse MCDR2 gene being provided.
(FR) L'invention concerne une souris knock-out du gène MCDR2 obtenue à l'aide d'un système CRISPR-Cas9 pour modifier un gène MCDR2 et réaliser un transfert nucléaire sur cellule somatique. De l'ARNsg est conçu pour la région CDS du gène MCDR2 de souris et est clivé au moyen du système CRISPR-Cas9 pour obtenir une inactivation par knock-out du gène MCDR2, puis des individus murins knock-out correspondants sont obtenus, l'invention concernant également une méthode pratique pour l'étude du gène MCDR2 de souris.
(ZH) 提供一种利用CRISPR-Cas9系统对MCDR2基因进行编辑,并通过体细胞核移植技术获得MCDR2基因敲除的小鼠。针对小鼠MCDR2基因的CDS区设计sgRNA,并利用CRISPR-Cas9系统对其进行切割,实现了MCDR2基因的敲除,并且获得了相应的敲除小鼠个体,为研究小鼠MCDR2基因提供了一种切实可行的方法。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)