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1. WO2020000459 - CRISPR/CAS9-TARGETED KNOCKOUT OF HUMAN TP55 GENE AND SPECIFIC GRNA THEREFOR

Publication Number WO/2020/000459
Publication Date 02.01.2020
International Application No. PCT/CN2018/093869
International Filing Date 29.06.2018
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
113
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/113 (2010.01)
CPC
C12N 15/113
Applicants
  • 深圳市博奥康生物科技有限公司 SHENZHEN BIOCAN TECHNOLOGIES CO., LTD. [CN/CN]; 中国广东省深圳市 南山区西丽街道珠光路134号珠光创新科技园1栋509室 毛吉炎 MAO, Jiyan Room 509, Building 1, Zhuguang Innovation Park No.134 Zhuguang Road, Xilii Street, Nanshan District Shenzhen, Guangdong 518055, CN
Inventors
  • 毛吉炎 MAO, Jiyan; CN
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) CRISPR/CAS9-TARGETED KNOCKOUT OF HUMAN TP55 GENE AND SPECIFIC GRNA THEREFOR
(FR) INACTIVATION CIBLÉE PAR CRISPR/CAS9 DE GÈNE TP55 HUMAIN ET ARNG SPÉCIFIQUE ASSOCIÉ
(ZH) CRISPR/Cas9靶向敲除人Tp55基因的方法及其特异性gRNA
Abstract
(EN)
Provided are a method for CRISPR/Cas9-targeted knockout of a human Tp55 gene, and specific gRNA therefor. The method comprises: designing two target sites at upstream and downstream of human Tp55 gene according to the design principle of CRISPR/Cas9, synthesizing a corresponding oligonucleotide sequence, connecting the sequence to a px459 vector to construct a recombinant plasmid, and transfecting the recombinant plasmid to a human esophageal carcinoma cell strain to specifically knock the human Tp55 gene out.
(FR)
L'invention concerne une méthode pour l'inactivation ciblée par CRISPR/Cas9 d'un gène Tp55 humain, et un ARNg spécifique associé. La méthode comprend : la conception de deux sites cibles en amont et en aval du gène Tp55 humain selon le principe de conception de CRISPR/Cas9, la synthèse d'une séquence oligonucléotidique correspondante, la liaison de la séquence à un vecteur px459 pour construire un plasmide recombinant, et la transfection du plasmide recombinant dans une souche de cellule de carcinome œsophagien humain pour inactiver spécifiquement le gène Tp55 humain.
(ZH)
提供一种CRISPR/Cas9靶向敲除人Tp55基因的方法及其特异性gRNA,所述方法包括:根据CRISPR/Cas9的设计原则,在人Tp55基因的上下游设计两个靶位点,合成相应的寡核苷酸序列,连接至px459载体上构建重组质粒,将重组质粒转染人食管癌细胞株,特异性敲除人Tp55基因。
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