Processing

Please wait...

Settings

Settings

1. WO2020000454 - PLASMID EXPRESSING LWCAS13A GENE, CONSTRUCTION METHOD THEREFOR AND USE THEREOF

Publication Number WO/2020/000454
Publication Date 02.01.2020
International Application No. PCT/CN2018/093864
International Filing Date 29.06.2018
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
64
General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
C12N 15/63 (2006.01)
C12N 15/64 (2006.01)
CPC
C12N 15/63
C12N 15/64
Applicants
  • 深圳市博奥康生物科技有限公司 SHENZHEN BIOCAN TECHNOLOGIES CO., LTD. [CN/CN]; 中国广东省深圳市 南山区西丽街道珠光路134号珠光创新科技园1栋509室毛吉炎 MAO, Jiyan Room 509, Building 1, Zhuguang Innovation Park No.134 Zhuguang Road, Xilii Street, Nanshan District Shenzhen, Guangdong 518055, CN
Inventors
  • 毛吉炎 MAO, Jiyan; CN
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) PLASMID EXPRESSING LWCAS13A GENE, CONSTRUCTION METHOD THEREFOR AND USE THEREOF
(FR) PLASMIDE EXPRIMANT LE GÈNE LWCAS13A, SA MÉTHODE DE CONSTRUCTION ET SON UTILISATION
(ZH) 一种表达LwCas13a基因的质粒、构建方法及其应用
Abstract
(EN)
Provided is a plasmid pRNAT-LwCas13a-Neo expressing the LwCas13a gene. The plasmid is constructed by the recombination of the CDS sequence of the LwCas13a gene and the pRNAT-U6.1/Neo eukaryotic expression vector. The nucleotide sequence thereof is as shown in SEQ ID NO.1. Also provided are a method for constructing the plasmid expressing the LwCas13a gene and the use thereof. The plasmid expressing the LwCas13a gene provided herein can replace the existing plasmid for RNA interference, and can induce the production of RNA interference with a higher specificity and lower off-target rate in cells.
(FR)
L'invention concerne un plasmide pRNAT-LwCas13a-Neo exprimant le gène LwCas13a. Le plasmide est construit par recombinaison de la séquence CDS du gène LwCas13a et du vecteur d'expression eucaryote pRNAT-U6.1/Neo. La séquence nucléotidique de celui-ci est telle que représentée dans la SEQ ID NO. 1. L'invention concerne également une méthode de construction du plasmide exprimant le gène LwCas13a et son utilisation. Le plasmide exprimant le gène LwCas13a, selon l'invention, peut remplacer le plasmide existant pour l'interférence ARN, et peut induire la production d'une interférence ARN avec une spécificité plus élevée et un taux hors cible inférieur dans les cellules.
(ZH)
提供了一种表达LwCas13a基因的质粒pRNAT-LwCas13a-Neo,包括LwCas13a基因CDS序列与pRNAT-U6.1/Neo真核表达载体的重组构建而成,其核苷酸序列如SEQ ID NO.1所示。还提供了一种表达LwCas13a基因质粒的构建方法及其应用。所提供的表达LwCas13a基因的质粒可替代现有的用于RNA干扰的质粒,可在细胞中诱导产生特异性更高、脱靶率更低的RNA干扰。
Latest bibliographic data on file with the International Bureau