WO2019243714 METHOD FOR DETECTING BACTERIA ACCORDING TO THE GRAM SIGNAL THEREOF IN A COMPLEX SAMPLE

Settings

Stemming

Single Family Member

Include NPL

Feedback

Goto Application

Publication Number WO/2019/243714

Publication Date 26.12.2019

International Application No. PCT/FR2019/051435

International Filing Date 13.06.2019

IPC

CPC

Applicants

MAAT PHARMA [FR]/[FR]
BIOASTER [FR]/[FR]

Inventors

DUQUENOY, Aurore
BELLAIS, Samuel
THOMAS, Vincent

Agents

SANTARELLI

Priority Data

1855350

18.06.2018

Publication Language French (fr)

Filing Language French (FR)

Designated States

View all

Title

(EN) METHOD FOR DETECTING BACTERIA ACCORDING TO THE GRAM SIGNAL THEREOF IN A COMPLEX SAMPLE

(FR) PROCÉDÉ DE DÉTECTION DES BACTÉRIES SELON LEUR SIGNAL GRAM DANS UN ÉCHANTILLON COMPLEXE

Abstract

(EN) The invention relates to a method for detecting the proportion of Gram-positive and Gram-negative bacteria in a complex sample. The bacteria are detected according to the Gram signal thereof. The invention enables distinction between species of Gram-positive bacteria and species of Gram-negative bacteria in a complex sample. The invention also relates to a kit for marking Gram-positive and Gram-negative bacteria, particularly for use in flow cytometry, particularly for complex samples such as human or animal microbiota samples.

(FR) La présente invention concerne un procédé de détection de la proportion de bactéries à Gram positif et à Gram négatif dans un échantillon complexe. La détection des bactéries se fait selon leur signal Gram. L'invention permet de distinguer des espèces de bactéries à Gram positif et à Gram négatif dans un échantillon complexe. Elle concerne également un kit de marquage des bactéries à Gram positif et à Gram négatif, notamment pour utilisation en cytométrie en flux, notamment pour des échantillons complexes comme des échantillons de microbiote - humain ou animal.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02	involving viable microorganisms
04	Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
569	for microorganisms, e.g. protozoa, bacteria, viruses

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02	involving viable microorganisms
04	Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
569	for microorganisms, e.g. protozoa, bacteria, viruses

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02	involving viable microorganisms
04	Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Processing

National Phase Entries

Authority File

Settings

Feedback

Goto Application

1. WO2019243714 - METHOD FOR DETECTING BACTERIA ACCORDING TO THE GRAM SIGNAL THEREOF IN A COMPLEX SAMPLE

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333	Assays involving biological materials from specific organisms or of a specific nature
435	from animals; from humans
46	from vertebrates
47	Assays involving proteins of known structure or function as defined in the subgroups
4701	Details
4724	Lectins

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333	Assays involving biological materials from specific organisms or of a specific nature
435	from animals; from humans
46	from vertebrates
47	Assays involving proteins of known structure or function as defined in the subgroups
4701	Details
4724	Lectins