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1. WO2019219024 - USE OF CRRNA-MEDIATED CRISPR/CAS13A GENE EDITING SYSTEM IN TUMOR CELLS

Publication Number WO/2019/219024
Publication Date 21.11.2019
International Application No. PCT/CN2019/087019
International Filing Date 15.05.2019
IPC
C12N 15/90 2006.1
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
C12N 15/113 2010.1
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
CPC
C12N 15/113
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
C12N 15/907
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
902using homologous recombination
907in mammalian cells
C12N 2310/10
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2310Structure or type of the nucleic acid
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C12N 2310/20
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2310Structure or type of the nucleic acid
10Type of nucleic acid
20involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Applicants
  • 康春生 KANG, Chunsheng [CN]/[CN]
Inventors
  • 康春生 KANG, Chunsheng
  • 王琦雪 WANG, Qixue
  • 周俊虎 ZHOU, Junhu
Agents
  • 上海光华专利事务所(普通合伙) J.Z.M.C. PATENT AND TRADEMARK LAW OFFICE (GENERAL PARTNERSHIP)
Priority Data
201810465791.316.05.2018CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) USE OF CRRNA-MEDIATED CRISPR/CAS13A GENE EDITING SYSTEM IN TUMOR CELLS
(FR) UTILISATION D'UN SYSTÈME D'ÉDITION DE GÈNE CRISPR/CAS13A À MÉDIATION PAR ARNCR DANS DES CELLULES TUMORALES
(ZH) crRNA介导的CRISPR/Cas13a基因编辑系统在肿瘤细胞中的应用
Abstract
(EN) Provided is use of a crRNA-mediated CRISPR/Cas13a gene editing system in inhibiting and killing tumor cells. Cas13a protein, in U87 glioma cell line, can be mediated by a single-stranded crRNA onto a complementary RNA of interest and perform cleavage. Meanwhile, the Cas13a protein can also trigger the effect of associated cleavage in eukaryotic cells, that is, after starting to cleave the first RNA of interest, the Cas13a protein performs a random, non-targeted cleavage on other encountered RNA, thereby achieving effects of inhibiting and killing tumors such as reducing tumor cell index, and inhibiting the tumor formation rate and tumor size of mice.
(FR) L'invention concerne l'utilisation d'un système d'édition de gène CRISPR/Cas13a à médiation par ARNcr pour inhiber et tuer des cellules tumorales. La protéine Cas13a, dans lignée cellulaire de gliome U87, peut être médiée par un ARNcr monobrin sur un ARN complémentaire d'intérêt et effectuer un clivage. Pendant ce temps, la protéine Cas13a peut également déclencher l'effet d'un clivage associé dans des cellules eucaryotes, c'est-à-dire après l'initiation du clivage du premier ARN d'intérêt, la protéine Cas13a réalise un clivage aléatoire, non ciblé sur d'autres ARN rencontrés, ce qui permet d'obtenir des effets d'inhibition et de destruction de tumeurs tels que la réduction de l'indice de cellules tumorales et l'inhibition du taux de formation tumorale et la taille tumorale de souris.
(ZH) 提供了一种crRNA介导的CRISPR/Cas13a基因编辑系统在抑制和杀伤肿瘤细胞中的应用。其中Cas13a蛋白在U87胶质瘤细胞系中,可以由单链的crRNA介导到互补的目的RNA上,并进行切割。同时,Cas13a蛋白还能够在真核生物细胞中触发连带剪切的效应,即在开始切割第一条目的RNA之后,Cas13a蛋白对其他遇到的RNA,进行无目的的随机切割,从而起到降低肿瘤细胞指数,抑制小鼠成瘤率和肿瘤大小等抑制和杀伤肿瘤的作用。
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