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1. WO2019211958 - TRANSFORMANT AND PRODUCTION METHOD FOR ORGANIC COMPOUND USING SAME

Publication Number WO/2019/211958
Publication Date 07.11.2019
International Application No. PCT/JP2019/013368
International Filing Date 27.03.2019
IPC
C12N 1/21 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 15/53 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
C12N 15/60 2006.1
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
60Lyases (4)
C12P 7/02 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
C12P 7/40 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
40containing a carboxyl group
C12P 13/04 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta-amino acids
CPC
C12N 15/52
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
C12N 15/77
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
77for Corynebacterium; for Brevibacterium
C12N 9/0006
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0006acting on CH-OH groups as donors (1.1)
C12N 9/88
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9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
C12P 13/06
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PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta- amino acids
06Alanine; Leucine; Isoleucine; Serine; Homoserine
C12P 7/065
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
06Ethanol, i.e. non-beverage
065with microorganisms other than yeasts
Applicants
  • 公益財団法人地球環境産業技術研究機構 RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH [JP]/[JP]
Inventors
  • 乾将行 INUI Masayuki
  • 須田雅子 SUDA Masako
  • 加藤直人 KATO Naoto
  • 長谷川智 HASEGAWA Satoshi
  • 小暮高久 KOGURE Takahisa
  • 城島透 JOJIMA Toru
Agents
  • 特許業務法人池内アンドパートナーズ IKEUCHI & PARTNERS
Priority Data
2018-08842501.05.2018JP
Publication Language Japanese (ja)
Filing Language Japanese (JA)
Designated States
Title
(EN) TRANSFORMANT AND PRODUCTION METHOD FOR ORGANIC COMPOUND USING SAME
(FR) TRANSFORMANT ET PROCÉDÉ DE PRODUCTION DE COMPOSÉ ORGANIQUE UTILISANT CELUI-CI
(JA) 形質転換体及びそれを用いた有機化合物の製造方法
Abstract
(EN) Provided is a method for improving the efficiency of production of an organic compound by a bacterium that does not inherently have an endogenous ED pathway. One embodiment pertains to a transformant of a coryneform bacterium obtained by introducing the Entner-Doudoroff pathway into a host coryneform bacterium. Another embodiment pertains to a transformant of a coryneform bacterium obtained by introducing, into a host coryneform bacterium, a gene encoding an enzyme having glucose-6-phosphate dehydrogenase activity, a gene encoding an enzyme having 6-phosphogluconate dehydratase activity, and a gene encoding an enzyme having 2-keto-3-deoxy-6-phosphogluconate aldolase activity.
(FR) L'invention concerne un procédé pour améliorer l'efficacité de production d'un composé organique par une bactérie qui ne comprend pas de manière inhérente de voie ED endogène. Un mode de réalisation concerne un transformant d'une bactérie corynéforme obtenu par l'introduction de la voie d'Entner-Doudoroff dans une bactérie corynéforme hôte. Un autre mode de réalisation concerne un transformant d'une bactérie corynéforme obtenu par l'introduction, dans une bactérie corynéforme hôte, d'un gène codant pour une enzyme ayant une activité de glucose-6-phosphate déshydrogénase, d'un gène codant pour une enzyme ayant une activité de 6-phosphogluconate déshydratase, et d'un gène codant pour une enzyme ayant une activité de 2-céto-3-désoxy-6-phosphogluconate aldolase.
(JA) 本来内在するED経路を持たない細菌における有機化合物の生産性向上方法の提供。 一態様において、宿主のコリネ型細菌にエントナー-ドゥドロフ経路が導入された、コリネ型細菌の形質転換体に関する。その他の一態様において、宿主のコリネ型細菌に、グルコース6-リン酸デヒドロゲナーゼ活性を有する酵素がコードされた遺伝子、6-ホスホグルコン酸デヒドラターゼ活性を有する酵素がコードされた遺伝子、及び2-ケト-3-デオキシ-6-ホスホグルコン酸アルドラーゼ活性を有する酵素がコードされた遺伝子が導入された、コリネ型細菌の形質転換体に関する。
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