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1. WO2019211749 - THERMOSTABLE VIRAL REVERSE TRANSCRIPTASE

Publication Number WO/2019/211749
Publication Date 07.11.2019
International Application No. PCT/IB2019/053537
International Filing Date 30.04.2019
IPC
C07K 14/005 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
C12N 9/12 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
CPC
C07K 14/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C07K 14/005
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
C12N 2795/00022
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2795Bacteriophages
00011Bacteriophages
00022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
C12N 9/1241
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1241Nucleotidyltransferases (2.7.7)
C12N 9/1276
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1241Nucleotidyltransferases (2.7.7)
1276RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
Applicants
  • QIAGEN BEVERLY, LLC [US]/[US]
Inventors
  • SCHOENFELD, Thomas William
  • HELLER, Ryan Charles
  • CRISSY, Katarzyna
  • CHUNG, Suhman
Agents
  • POHLMAN, Sandra
Priority Data
18173195.118.05.2018EP
62/665,56002.05.2018US
62/790,48310.01.2019US
62/835,52118.04.2019US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) THERMOSTABLE VIRAL REVERSE TRANSCRIPTASE
(FR) TRANSCRIPTASE INVERSE VIRALE THERMOSTABLE
Abstract
(EN) The present invention provides novel engineered polypeptides that support both reverse transcription and DNA amplification in manganese-independent reactions. The present invention also provides methods for amplifying template nucleic acids using such polypeptides. This invention addresses deficiencies in the current state of the art in nucleic acid amplification-based detection of template nucleic acids, especially RNA targets, including deficiencies in detection sensitivity, specificity, enzyme stability, inhibitor tolerance and time to result compared with manganese-dependent thermostable reverse transcriptases and two-enzyme solutions.
(FR) La présente invention concerne de nouveaux polypeptides modifiés qui supportent à la fois la transcription inverse et l'amplification d'ADN dans des réactions indépendantes du manganèse. La présente invention concerne également des procédés d'amplification d'acides nucléiques modèles à l'aide de tels polypeptides. La présente invention traite des déficiences dans l'état actuel de la technique se rapportant à la détection d'acides nucléiques modèles basée sur l'amplification d'acides nucléiques, en particulier de cibles d'ARN, notamment des déficiences dans la sensibilité de détection, la spécificité, la stabilité enzymatique, la tolérance aux inhibiteurs et le temps par comparaison avec des transcriptases inverses thermostables dépendantes du manganèse et des solutions à deux enzymes.
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