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1. WO2019211625 - IDENTIFICATION OF IMMUNOGLOBULINS USING MASS SPECTROMETRY

Publication Number WO/2019/211625
Publication Date 07.11.2019
International Application No. PCT/GB2019/051239
International Filing Date 03.05.2019
IPC
G01N 33/574 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 2800/52
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
52Predicting or monitoring the response to treatment; Prognosis
G01N 30/7266
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
62Detectors specially adapted therefor
72Mass spectrometers
7233interfaced to liquid or supercritical fluid chromatograph
724Nebulising, aerosol formation or ionisation
7266by electric field, e.g. electrospray
G01N 33/57426
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
57407Specifically defined cancers
57426leukemia
G01N 33/6851
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
6851Methods of protein analysis involving laser desorption ionisation mass spectrometry
G01N 33/6854
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6854Immunoglobulins
Applicants
  • THE BINDING SITE GROUP LIMITED [GB]/[GB]
Inventors
  • SAKRIKAR, Dhananjay
  • HARDING, Stephen
  • BARNIDGE, David
  • LAJKO, Michelle
Agents
  • WITHERS & ROGERS LLP
  • ELSY, David
Priority Data
1808529.024.05.2018GB
62/667,07604.05.2018US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) IDENTIFICATION OF IMMUNOGLOBULINS USING MASS SPECTROMETRY
(FR) IDENTIFICATION D'IMMUNOGLOBULINES PAR SPECTROMÉTRIE DE MASSE
Abstract
(EN) This document relates to materials and methods for identifying and/or quantifying immunoglobulins from a biological sample without pre-purification of the immunoglobulins prior to ionization and detection using mass spectrometry. For example, a monoclonal light chain from a monoclonal immunoglobulin may be observed using matrix assisted laser desorption ionization - time of flight (MALDI-TOF) mass spectrometry after diluting a sample containing the monoclonal immunoglobulin with an aqueous buffer containing acid and a reducing agent then mixing the sample with alpha- cyano-4-hydroxycinnamic acid matrix (CHCA). In another example, an intact monoclonal immunoglobulin may be observed in a sample using MALDI-TOF mass spectrometry after diluting the sample containing the monoclonal immunoglobulin with water then mixing the sample with CHCA matrix.
(FR) Le présent document concerne des matériaux et des procédés pour identifier et/ou quantifier des immunoglobulines à partir d'un échantillon biologique sans prépurification des immunoglobulines avant l'ionisation et la détection à l'aide de la spectrométrie de masse. Par exemple, une chaîne légère monoclonale provenant d'une immunoglobuline monoclonale peut être observée par spectrométrie de masse à temps de vol par désorption/ionisation laser assistée par matrice (MALDI-TOF) après dilution d'un échantillon contenant l'immunoglobuline monoclonale avec un tampon aqueux contenant de l'acide et un agent réducteur, puis en mélangeant l'échantillon avec une matrice d'acide alpha-cyano-4-hydroxycinnamique (CHCA). Dans un autre exemple, une immunoglobuline monoclonale intacte peut être observée dans un échantillon à l'aide d'une spectrométrie de masse MALDI-TOF après dilution de l'échantillon contenant l'immunoglobuline monoclonale avec de l'eau, puis en mélangeant l'échantillon avec la matrice CHCA.
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