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1. WO2019191061 - CELL IMAGING SYSTEMS AND METHODS

Publication Number WO/2019/191061
Publication Date 03.10.2019
International Application No. PCT/US2019/024025
International Filing Date 26.03.2019
IPC
G01B 9/02 2006.01
GPHYSICS
01MEASURING; TESTING
BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
9Instruments as specified in the subgroups and characterised by the use of optical measuring means
02Interferometers
G01N 21/45 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
41Refractivity; Phase-affecting properties, e.g. optical path length
45using interferometric methods; using Schlieren methods
G01N 21/55 2014.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
55Specular reflectivity
G06F 17/14 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
FELECTRIC DIGITAL DATA PROCESSING
17Digital computing or data processing equipment or methods, specially adapted for specific functions
10Complex mathematical operations
14Fourier, Walsh or analogous domain transformations
CPC
G01N 2021/418
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
41Refractivity; Phase-affecting properties, e.g. optical path length
4173Phase distribution
418Frequency/phase diagrams
G01N 21/41
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
41Refractivity; Phase-affecting properties, e.g. optical path length
G01N 2201/061
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2201Features of devices classified in G01N21/00
06Illumination; Optics
061Sources
G01N 2201/062
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2201Features of devices classified in G01N21/00
06Illumination; Optics
062LED's
G01N 2201/0826
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2201Features of devices classified in G01N21/00
08Optical fibres; light guides
0826Fibre array at source, distributing
G01N 33/4833
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
4833of solid biological material, e.g. tissue samples, cell cultures
Applicants
  • GEORGIA TECH RESEARCH CORPORATION [US]/[US]
Inventors
  • ROBLES, Francisco
  • LEDWIG, Patrick
Agents
  • WEEKS, Dustin B.
  • SCHNEIDER, Ryan A.
Priority Data
62/648,18026.03.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CELL IMAGING SYSTEMS AND METHODS
(FR) SYSTÈMES ET PROCÉDÉS D'IMAGERIE CELLULAIRE
Abstract
(EN)
Disclosed herein are systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The disclosed methods and systems can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.
(FR)
L'invention concerne des systèmes et des procédés d'imagerie cellulaire. L'imagerie en phase quantitative, QPI, utilise des variations dans l'indice de réfraction d'un échantillon en tant que source de contraste endogène, fournissant des informations sans marqueur de structures sous-cellulaires et permettant la reconstruction de paramètres biophysiques précieux, telle qu'une masse sèche cellulaire à des échelles du femtogramme, un transport de masse, et une épaisseur d'échantillon et des fluctuations à des échelles du nanomètre. En conséquence, la QPI est devenu un outil précieux en biologie et en médecine. Cependant, la QPI a souffert de la nécessité d'un trans-éclairage à travers des objets relativement minces afin d'avoir accès au champ diffusé vers l'avant, qui transporte des informations fréquentielles spatiales faibles cruciales d'un échantillon et évite des contributions de multiples plans de lumière diffusée ou hors foyer. Les procédés et systèmes selon l'invention peuvent fournir une reconstruction de la QPI et une analyse correspondante pour l'imagerie d'échantillons de cellules dans des échantillons épais à l'aide d'une configuration d'épi-éclairage.
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