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1. WO2019165148 - DETERMINING CONDITIONS FOR PURIFICATION OF PROTEINS

Publication Number WO/2019/165148
Publication Date 29.08.2019
International Application No. PCT/US2019/019050
International Filing Date 21.02.2019
IPC
B01D 15/16 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08Selective adsorption, e.g. chromatography
10characterised by constructional or operational features
16relating to the conditioning of the fluid carrier
G01N 30/86 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
86Signal analysis
CPC
B01D 15/166
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
10characterised by constructional or operational features
16relating to the conditioning of the fluid carrier
166Fluid composition conditioning, e.g. gradient
G01N 2030/8831
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
88Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
8809analysis specially adapted for the sample
8813biological materials
8831involving peptides or proteins
G01N 2030/889
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
88Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
889monitoring the quality of the stationary phase; column performance
G01N 30/8658
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
86Signal analysis
8658Optimising operation parameters
G01N 30/8693
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
86Signal analysis
8693Models, e.g. prediction of retention times, method development and validation
G01N 30/88
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
88Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
Applicants
  • JUST BIOTHERAPEUTICS, INC. [US]/[US]
Inventors
  • SHAVER, Jeremy, Martin
  • AMIMEUR, Tileli
  • GILLESPIE, Ron
  • KETCHEM, Randal, R.
  • GARCIA, Fernando
Agents
  • ARORA, Suneel
Priority Data
62/633,58421.02.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DETERMINING CONDITIONS FOR PURIFICATION OF PROTEINS
(FR) DÉTERMINATION DE CONDITIONS DE PURIFICATION DE PROTÉINES
Abstract
(EN)
Technologies are described related to determining conditions for the purification of proteins. In some implementations, models can be generated that predict yield and purity for various chromatographic techniques at a number of pH levels and salt concentrations. Training data used to produce the models can be obtained by running chromatography columns using stationary phase materials of different chromatographic techniques at various combinations of pH levels and salt concentrations. In additional implementations, the training data can be obtained by analyzing solutions included in a subset of wells of a multi-well plate, where the subset of wells are associated with particular salt concentrations and pH values for a particular stationary phase material. Further, the models can be used to determine optimized conditions for the purification of various proteins based on maximizing yield and purity, while minimizing cost.
(FR)
L'invention concerne des technologies associées à la détermination d'états pour la purification de protéines. Dans certains modes de réalisation, des modèles peuvent être générés qui prédisent le rendement et la pureté pour diverses techniques chromatographiques à un certain nombre de niveaux de pH et de concentrations de sel. Des données d'apprentissage utilisées pour produire les modèles peuvent être obtenues par exécution de colonnes de chromatographie à l'aide de matériaux de phase stationnaires de différentes techniques chromatographiques à diverses combinaisons de niveaux de pH et de concentrations de sel. Dans d'autres modes de réalisation, les données d'apprentissage peuvent être obtenues par analyse de solutions incluses dans un sous-ensemble de puits d'une plaque multipuits, le sous-ensemble de puits étant associé à des concentrations particulières de sel et à des valeurs de pH pour un matériau de phase stationnaire particulier. En outre, les modèles peuvent être utilisés pour déterminer des conditions optimisées pour la purification de diverses protéines sur la base de la maximisation du rendement et de la pureté, tout en réduisant au minimum le coût.
Also published as
EP2019710843
Latest bibliographic data on file with the International Bureau