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1. (WO2019165148) DETERMINING CONDITIONS FOR PURIFICATION OF PROTEINS
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/165148 International Application No.: PCT/US2019/019050
Publication Date: 29.08.2019 International Filing Date: 21.02.2019
IPC:
B01D 15/16 (2006.01) ,G01N 30/86 (2006.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
15
Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08
Selective adsorption, e.g. chromatography
10
characterised by constructional or operational features
16
relating to the conditioning of the fluid carrier
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30
Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02
Column chromatography
86
Signal analysis
Applicants:
JUST BIOTHERAPEUTICS, INC. [US/US]; 401 Terry Ave North Seattle, WA 98109, US
Inventors:
SHAVER, Jeremy, Martin; US
AMIMEUR, Tileli; US
GILLESPIE, Ron; US
KETCHEM, Randal, R.; US
GARCIA, Fernando; US
Agent:
ARORA, Suneel; US
Priority Data:
62/633,58421.02.2018US
Title (EN) DETERMINING CONDITIONS FOR PURIFICATION OF PROTEINS
(FR) DÉTERMINATION DE CONDITIONS DE PURIFICATION DE PROTÉINES
Abstract:
(EN) Technologies are described related to determining conditions for the purification of proteins. In some implementations, models can be generated that predict yield and purity for various chromatographic techniques at a number of pH levels and salt concentrations. Training data used to produce the models can be obtained by running chromatography columns using stationary phase materials of different chromatographic techniques at various combinations of pH levels and salt concentrations. In additional implementations, the training data can be obtained by analyzing solutions included in a subset of wells of a multi-well plate, where the subset of wells are associated with particular salt concentrations and pH values for a particular stationary phase material. Further, the models can be used to determine optimized conditions for the purification of various proteins based on maximizing yield and purity, while minimizing cost.
(FR) L'invention concerne des technologies associées à la détermination d'états pour la purification de protéines. Dans certains modes de réalisation, des modèles peuvent être générés qui prédisent le rendement et la pureté pour diverses techniques chromatographiques à un certain nombre de niveaux de pH et de concentrations de sel. Des données d'apprentissage utilisées pour produire les modèles peuvent être obtenues par exécution de colonnes de chromatographie à l'aide de matériaux de phase stationnaires de différentes techniques chromatographiques à diverses combinaisons de niveaux de pH et de concentrations de sel. Dans d'autres modes de réalisation, les données d'apprentissage peuvent être obtenues par analyse de solutions incluses dans un sous-ensemble de puits d'une plaque multipuits, le sous-ensemble de puits étant associé à des concentrations particulières de sel et à des valeurs de pH pour un matériau de phase stationnaire particulier. En outre, les modèles peuvent être utilisés pour déterminer des conditions optimisées pour la purification de diverses protéines sur la base de la maximisation du rendement et de la pureté, tout en réduisant au minimum le coût.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)