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1. WO2019149963 - METHODS FOR DETECTING FUNGI IN TURF GRASS WITH A LAMP ASSAY HAVING NOVEL PRIMER SETS

Publication Number WO/2019/149963
Publication Date 08.08.2019
International Application No. PCT/EP2019/052803
International Filing Date 05.02.2019
IPC
C12Q 1/6895 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
6895for plants, fungi or algae
CPC
C12Q 1/6895
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
6895for plants, fungi or algae
C12Q 2527/101
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2527Reactions demanding special reaction conditions
101Temperature
C12Q 2600/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
16Primer sets for multiplex assays
Applicants
  • SYNGENTA PARTICIPATIONS AG [CH]/[CH]
Inventors
  • PALMISANO, Marilena
  • WOHLER, Christian
Agents
  • SYNGENTA INTERNATIONAL AG
Priority Data
18155093.005.02.2018EP
18159821.002.03.2018EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR DETECTING FUNGI IN TURF GRASS WITH A LAMP ASSAY HAVING NOVEL PRIMER SETS
(FR) PROCÉDÉS DE DÉTECTION DE CHAMPIGNONS DANS L'HERBE À GAZON AVEC UN TEST LAMP COMPRENANT DE NOUVEAUX ENSEMBLES D'AMORCES
Abstract
(EN) The present invention provides a method for detecting fungal DNA in a turf grass sample with a loop- mediated isothermal amplification (LAMP) assay which contains primers for fungal DNA of at least one turf pathogenic fungi selected from Sclerotinia homoeocarpa, Rhizoctonia solani spp., Pythium aphanidermatum, Gaeumannomyces graminis spp., Microdochium nivale spp., Magnaporthe poae, Colletotrichum graminicola, Colletotrichum cereale and Pythium ultimum var. ultimum, comprising: subjecting the turf sample to a LAMP reaction wherein the LAMP reaction uses a primer set of four or more nucleic acid sequences with each primer in the set having from 15 to 50 nucleic acids The primers useful in the present method are selected from specifically selected internal transcribed spacer regions or genes of the target fungi to provide improved assay results.
(FR) La présente invention concerne un procédé de détection d'ADN fongique dans un échantillon d'herbe à gazon avec un test par amplification isotherme dédiée par boucle (LAMP) qui contient des amorces pour l'ADN fongique d'au moins un champignon pathogène de gazon sélectionné parmi Sclerotinia homoeocarpa, Rhizoctonia solani spp, Pythium aphanidermatum, Gaeumannomyces graminis spp, Microdochium nivale spp, Magnaporthe poae, Colletotrichum graminicola, Colletotrichum cereale et Pythium ultimum var. ultimum, comprenant les étapes consistant à : soumettre l'échantillon de gazon à une réaction LAMP, la réaction LAMP utilisant un ensemble d'amorces de quatre séquences d'acides nucléiques ou plus, chaque amorce dans l'ensemble contenant de 15 à 50 acides nucléiques. Les amorces utiles dans le présent procédé sont choisies dans des régions d'espaceur transcrites internes spécifiquement sélectionnées ou des gènes des champignons cibles pour fournir des résultats de test améliorés.
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