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1. (WO2019067982) PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS
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Pub. No.: WO/2019/067982 International Application No.: PCT/US2018/053546
Publication Date: 04.04.2019 International Filing Date: 28.09.2018
IPC:
C07K 14/005 (2006.01) ,C12N 7/00 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005
from viruses
01
DNA viruses
03
Herpetoviridae, e.g. pseudorabies virus
05
Epstein-Barr virus
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7
Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
Applicants:
MASSACHUSETTS EYE AND EAR INFIRMARY [US/US]; 243 Charles Street Boston, Massachusetts 02114, US
THE SCHEPENS EYE RESEARCH INSTITUTE, INC. [US/US]; 20 Staniford Street Boston, Massachusetts 02114, US
UNIVERSITE DE NANTES [CH/FR]; 1 quai de Tourville 44000 Nantes, FR
CENTRE HOSPITALIER UNIVERSITAIRE DE NANTES [FR/FR]; 5 allée de l'Ile Gloriette 44000 Nantes, FR
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE [FR/FR]; 10 Rue De Tolbiac 75013 Paris, FR
Inventors:
AYUSO, Eduard; FR
ROBIN, Cecile; FR
PENAUD-BUDLOO, Magalie; FR
FRANCOIS, Achille; FR
BLOUIN, Véronique; FR
VANDENBERGHE, Luk H.; US
MAURER, Anna Claire; US
Agent:
FASSE, J. Peter; US
PARSONS, M. Angela; US
Priority Data:
62/565,85129.09.2017US
Title (EN) PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS
(FR) PRODUCTION DE VIRUS ADÉNO-ASSOCIÉS DANS DES CELLULES D'INSECTES
Abstract:
(EN) This disclosure relates to the field of high scale production of recombinant Adeno-Associated Viruses (AAVs). The inventors have conceived of specific nucleic acid constructs that allow for high scale production of recombinant AAV particles in insect cells. Importantly, these nucleic constructs do not require the production of a heterologous AAP. This disclosure thus relates to a nucleic acid for producing AAV capsids in insect cells, where the nucleic acid includes a first open reading frame encoding the VP1, VP2, and VP3 proteins, and a second open reading frame encoding the Assembly-Activating Protein (AAP).
(FR) La présente divulgation concerne le domaine de la production à grande échelle de virus adéno-associés recombinés (AAV). Les inventeurs ont conçu des constructions d'acides nucléiques spécifiques qui permettent la production à grande échelle de particules d'AAV recombinées dans des cellules d'insectes. De manière importante, ces constructions nucléiques ne nécessitent pas la production d'une AAP hétérologue. La présente divulgation concerne par conséquent un acide nucléique pour la production de capsides d'AAV dans des cellules d'insectes, où l'acide nucléique comprend un premier cadre de lecture ouvert codant pour les protéines VP1, VP2 et VP3, et un second cadre de lecture ouvert codant pour la protéine d'activation de l'assemblage (AAP).
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)