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1. (WO2019066363) EXTRACTION DEVICE, EXTRACTION METHOD AND EXTRACTION SYSTEM FOR EXTRACTING TARGET SUBSTANCE
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Pub. No.: WO/2019/066363 International Application No.: PCT/KR2018/011026
Publication Date: 04.04.2019 International Filing Date: 19.09.2018
IPC:
B01L 3/00 (2006.01) ,C12N 15/10 (2006.01) ,C12Q 1/6806 (2018.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
L
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3
Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
[IPC code unknown for C12Q 1/6806]
Applicants:
고려대학교 산학협력단 KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION [KR/KR]; 서울시 성북구 안암로 145 145, Anam-ro Seongbuk-gu, Seoul 02841, KR
Inventors:
신세현 SHIN, Se-Hyun; KR
이호윤 LEE, Ho-Yoon; KR
나원휘 NA, Won-Hwi; KR
서창덕 SEO, Chang-Deok; KR
Agent:
특허법인 남촌 NAMCHON INTERNATIONAL PATENT AND LAW FIRM; 서울시 종로구 새문안로5길 37, 도렴빌딩 406호 (도렴동) (Doryeom-dong) 406 Doryeom Bldg., 37, Saemunan-ro 5-gil Jongno-gu, Seoul 03173, KR
Priority Data:
10-2017-012479827.09.2017KR
Title (EN) EXTRACTION DEVICE, EXTRACTION METHOD AND EXTRACTION SYSTEM FOR EXTRACTING TARGET SUBSTANCE
(FR) DISPOSITIF D'EXTRACTION, PROCÉDÉ D'EXTRACTION ET SYSTÈME D'EXTRACTION POUR EXTRAIRE UNE SUBSTANCE CIBLE
(KO) 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템
Abstract:
(EN) The present invention relates to an extraction device, an extraction method and an extraction system for extracting a target substance. The extraction device according to the present invention comprises: a sample kit; a porous membrane installed in the sample kit and having a target substance bound thereto; an elution buffer which is stacked over the porous membrane in the sample kit, passes through the porous membrane and thereby elutes the target substance out of the porous membrane; and a bipolar solution which has a different polarity from the elution buffer and is therefore not mixed therewith and is stacked over the elution buffer in the sample kit, wherein the elution buffer and the bipolar solution pass through the porous membrane in order, and, when passing through the porous membrane, the bipolar solution pushes out the elution buffer left in the porous membrane. Accordingly, a target substance such as a nucleic acid can be extracted even without using a centrifugation method or applying high-speed centrifugation.
(FR) La présente invention concerne un dispositif d'extraction, un procédé d'extraction et un système d'extraction pour extraire une substance cible. Le dispositif d'extraction selon la présente invention comprend : un kit d'échantillon; une membrane poreuse installée dans le kit d'échantillon et ayant une substance cible liée à celle-ci; un tampon d'élution qui est empilé sur la membrane poreuse dans le kit d'échantillon, passe à travers la membrane poreuse et élue ainsi la substance cible hors de la membrane poreuse; et une solution bipolaire qui a une polarité différente du tampon d'élution et, par conséquent, n'est pas mélangée à celui-ci et est empilée sur le tampon d'élution dans le kit d'échantillon, le tampon d'élution et la solution bipolaire passant à travers la membrane poreuse dans l'ordre et, lors du passage à travers la membrane poreuse, la solution bipolaire poussant le tampon d'élution restant dans la membrane poreuse. Par conséquent, une substance cible telle qu'un acide nucléique peut être extraite, même sans utiliser de procédé de centrifugation ou appliquer de centrifugation à grande vitesse.
(KO) 본 발명은 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템에 관한 것이다. 본 발명에 따른 추출 장치는 시료 키트와, 상기 시료 키트의 내부에 설치되어 상기 타겟 물질이 바인딩되는 다공막과, 상기 시료 키트 내에서 상기 다공막에 적층되어 상기 다공막을 통과하면서 상기 다공막으로부터 상기 타겟 물질을 용출하는 용출 용액과, 상기 용출 용액과 상이한 극성을 가져 상기 용출 용액과 섞이지 않는 상태로 상기 시료 키트 내에서 상기 용출 용액에 적층되는 이극성 용액을 포함하며; 상기 용출 용액과 상기 이극성 용액이 순차적으로 상기 다공막을 통과하고; 상기 이극성 용액이 상기 다공막을 통과할 때 상기 다공막에 잔존하는 상기 용출 용액을 밀어내는 것을 특징으로 한다. 이에 따라, 원심 분리 방식을 이용하지 않거나 고속의 원심 분리를 적용하지 않으면서도 핵산과 같은 타겟 물질을 추출할 수 있다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)