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1. (WO2019066249) METHOD FOR PRODUCING POLYCLONAL ANTIBODY AGAINST GIGANTEA PROTEIN OF ARABIDOPSIS THALIANA
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Pub. No.: WO/2019/066249 International Application No.: PCT/KR2018/009412
Publication Date: 04.04.2019 International Filing Date: 16.08.2018
IPC:
C07K 16/16 (2006.01) ,C07K 16/06 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
16
against material from plants
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
06
from serum
Applicants:
경상대학교산학협력단 INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY [KR/KR]; 경상남도 진주시 진주대로 501 501, Jinju-daero Jinju-si Gyeongsangnam-do 52828, KR
Inventors:
김외연 KIM, Woe-yeon; KR
차준영 CHA, Joon-Yung; KR
Agent:
최규환 CHOI, Kyu Whan; KR
Priority Data:
10-2017-012791629.09.2017KR
Title (EN) METHOD FOR PRODUCING POLYCLONAL ANTIBODY AGAINST GIGANTEA PROTEIN OF ARABIDOPSIS THALIANA
(FR) PROCÉDÉ DE PRODUCTION D'ANTICORPS POLYCLONAL CONTRE LA PROTÉINE GIGANTEA D'ARABIDOPSIS THALIANA
(KO) 애기장대 자이겐티아 단백질에 대한 다클론 항체의 제조방법
Abstract:
(EN) The present invention relates to a method of producing a polyclonal antibody against GIGANTEA protein, which comprises producing a cleaved peptide having an amino terminal of the GIGANTEA protein and a cleaved peptide having a carboxy terminal of the GIGANTEA protein in E. coli, and then mixing and injecting the produced recombinant cleaved peptides of GIGANTEA into a rabbit to produce a polyclonal antibody against the GIGANTEA protein; and a composition and a kit for detecting GIGANTEA protein comprising as an active ingredient a polyclonal antibody produced by the method.
(FR) La présente invention concerne un procédé de production d'un anticorps polyclonal contre la protéine GIGANTEA, qui consiste à produire un peptide clivé présentant une extrémité amino de la protéine GIGANTEA et un peptide clivé présentant une extrémité carboxy de la protéine GIGANTEA dans E. coli, puis à mélanger et à injecter les peptides clivés recombinants produits de GIGANTEA dans un lapin pour produire un anticorps polyclonal contre la protéine GIGANTEA; et une composition et un kit de détection de la protéine GIGANTEA comprenant, en tant que principe actif, un anticorps polyclonal produit par le procédé.
(KO) 본 발명은 자이겐티아 단백질의 아미노 말단과 카복시 말단의 절단체 펩티드를 대장균에서 각각 생산한 후, 생산된 재조합 자이겐티아 절단체 펩티드를 토끼에 혼합주사하여 자이겐티아 단백질에 대한 다클론 항체의 제조 방법, 상기 방법에 의해 제조된 다클론 항체를 유효성분으로 포함하는 자이겐티아 단백질 검출용 조성물 및 키트에 관한 것이다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)