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1. (WO2019064701) METHOD FOR COUNTING NUMBER OF MICROORGANISMS
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/064701 International Application No.: PCT/JP2018/021320
Publication Date: 04.04.2019 International Filing Date: 04.06.2018
IPC:
C12M 1/22 (2006.01) ,C12M 1/00 (2006.01) ,C12M 1/34 (2006.01) ,C12Q 1/04 (2006.01) ,C12N 1/20 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
22
Petri type dish
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
Applicants:
JNC CORPORATION [JP/JP]; 2-1, Otemachi 2-chome, Chiyoda-ku, Tokyo 1008105, JP
Inventors:
TERAMURA, Hajime; JP
FUJIWARA, Aoi; JP
Agent:
SANUKI, Shinichi; JP
NIWA, Takeshi; JP
KANKE, Hirohide; JP
ISAYAMA Masami; JP
Priority Data:
2017-19052229.09.2017JP
Title (EN) METHOD FOR COUNTING NUMBER OF MICROORGANISMS
(FR) PROCÉDÉ DE COMPTAGE DU NOMBRE DE MICRO-ORGANISMES
Abstract:
(EN) Provided is a method in which visibility of colonies of microorganisms in a medium is improved and quantitative counting of the number of microorganisms can be simply and accurately performed. The method of counting the number of microorganisms includes a step of adding a analyte to a composition containing (a) a polymer compound that can form non-flowable transparent gel without passing through dissolution by heating and without depending on cooling, (b) guar gum and (c) a nutritional ingredient and mixing the resulting mixture, a step of culturing the microorganisms contained in the analyte, and a step of counting the number of colonies of the microorganisms.
(FR) L'invention concerne un procédé dans lequel est améliorée la visibilité de colonies de micro-organismes dans un milieu et le comptage quantitatif du nombre de micro-organismes peut être effectué de manière simple et précise. Le procédé de comptage du nombre de micro-organismes comprend une étape consistant à ajouter un analyte à une composition contenant (a) un composé polymère qui peut former un gel transparent non fluide sans passer par une dissolution par chauffage et sans dépendre du refroidissement, (b) une gomme de guar et (c) un ingrédient nutritionnel et à mélanger le mélange ainsi obtenu, une étape consistant à cultiver les micro-organismes contenus dans l'analyte, et une étape consistant à compter le nombre de colonies des micro-organismes.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)