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1. (WO2019064463) METHOD AND DEVICE FOR CONCENTRATING VIRUS OR BACTERIUM USING BIOAFFINITY
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Pub. No.: WO/2019/064463 International Application No.: PCT/JP2017/035358
Publication Date: 04.04.2019 International Filing Date: 28.09.2017
IPC:
C12N 7/02 (2006.01) ,C12M 1/00 (2006.01) ,C12N 1/02 (2006.01) ,C07K 14/47 (2006.01) ,C07K 16/10 (2006.01) ,C07K 16/12 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7
Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
02
Recovery or purification
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
02
Separating micro-organisms from their culture media
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
46
from vertebrates
47
from mammals
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
08
against material from viruses
10
from RNA viruses
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
12
against material from bacteria
Applicants:
学校法人中部大学 CHUBU UNIVERSITY EDUCATIONAL FOUNDATION [JP/JP]; 愛知県春日井市松本町1200 1200, Matsumoto-cho, Kasugai-shi, Aichi 4878501, JP
株式会社ニデック NIDEK CO., LTD. [JP/JP]; 愛知県蒲郡市拾石町前浜34番地14 34-14, Maehama, Hiroishi-cho, Gamagori, Aichi 4430038, JP
Inventors:
上村 和秀 UEMURA Kazuhide; JP
河原 敏男 KAWAHARA Toshio; JP
小林 正彦 KOBAYASHI Masahiko; JP
成瀬 賢洋 NARUSE Masahiro; JP
藤井 智彦 FUJII Tomohiko; JP
Agent:
萩野 幹治 HAGINO Mikiharu; JP
Priority Data:
Title (EN) METHOD AND DEVICE FOR CONCENTRATING VIRUS OR BACTERIUM USING BIOAFFINITY
(FR) PROCÉDÉ ET DISPOSITIF POUR LA CONCENTRATION DE VIRUS OU DE BACTÉRIE PAR BIOAFFINITÉ
(JA) バイオアフィニティーを利用したウイルス又は細菌の濃縮方法及び装置
Abstract:
(EN) The present invention addresses the problem of providing a means for concentrating a virus or a bacterium simply and rapidly while maintaining the structure thereof. Provided is a method for concentrating a target virus or bacterium, the method characterized in that the target virus or bacterium is captured by a carrier via a metal ion-dependent ligand binding molecule, is isolated from the ligand binding molecule by treatment with a chelating agent, and is then recovered.
(FR) La présente invention a pour objet un moyen pour la concentration d'un virus ou d'une bactérie de manière simple et rapide tout en conservant la structure de celui-ci ou de celle-ci. À cet effet l'invention concerne un procédé pour la concentration d'un virus ou d'une bactérie cible, le procédé étant caractérisé en ce que le virus ou la bactérie cible est capturé(e) par un support par l'intermédiaire d'une molécule de liaison de type ligand dépendante d'un ion métallique, est isolé(e) de la molécule de liaison de type ligand par traitement avec un agent chélatant et est ensuite récupéré(e).
(JA) 本発明は、簡便且つ迅速に、しかもその構造の維持を図りつつ、ウイルスや細菌を濃縮する手段を提供することを課題とする。金属イオン依存性リガンド結合分子を介して標的のウイルス又は細菌を担体に捕捉した後、キレート剤で処理することによって前記リガンド結合分子から前記標的を脱離し、回収することを特徴とする、標的のウイルス又は細菌を濃縮する方法が提供される。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)