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1. (WO2019064268) A METHOD FOR EXPRESSION OF A PROKARYOTIC MEMBRANE PROTEIN IN AN EUKARYOTIC ORGANISM, PRODUCTS AND USES THEREOF
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Pub. No.: WO/2019/064268 International Application No.: PCT/IB2018/057572
Publication Date: 04.04.2019 International Filing Date: 28.09.2018
IPC:
C12N 15/62 (2006.01) ,C12N 15/81 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
62
DNA sequences coding for fusion proteins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
80
for fungi
81
for yeasts
Applicants:
UNIVERSIDADE DO MINHO [PT/PT]; Largo Do Paço 4704-553 Braga, PT
Inventors:
PEDRA AMORIM CASAL, Margarida Paula; PT
SOARES DA SILVA, Isabel João; PT
ALMEIDA PAIVA, Sandra Cristina; PT
NOGUEIRA RIBAS, David Manuel; PT
GOMES ALMEIDA, Ana Carolina; PT
Agent:
TEIXEIRA DE CARVALHO, Anabela; PT
Priority Data:
11031228.09.2017PT
Title (EN) A METHOD FOR EXPRESSION OF A PROKARYOTIC MEMBRANE PROTEIN IN AN EUKARYOTIC ORGANISM, PRODUCTS AND USES THEREOF
(FR) PROCÉDÉ D'EXPRESSION D'UNE PROTÉINE MEMBRANAIRE PROCARYOTE DANS UN ORGANISME EUCARYOTE, ET PRODUITS ET UTILISATIONS ASSOCIÉS
Abstract:
(EN) A method for the production of a functional prokaryotic membrane transporter protein in a eukaryotic host organism comprising the following steps: obtaining a DNA construct by ligating a DNA coding sequence of a prokaryotic membrane transporter protein to the N-terminal and/or C-terminal DNA coding sequences of a eukaryotic membrane protein; introducing the obtained DNA construct in the eukaryotic host organism for the production of the functional prokaryotic membrane transporter protein.
(FR) L'invention concerne un procédé de production d'une protéine fonctionnelle de transporteur membranaire procaryote dans un organisme hôte eucaryote comprenant les étapes suivantes : l'obtention d'une structure d'ADN par ligature d'une séquence de codage d'ADN d'une protéine de transport membranaire procaryote aux séquences de codage d'ADN de l'extrémité N- et/ou C-terminale d'une protéine membranaire eucaryote ; l'introduction de la structure d'ADN obtenue dans l'organisme hôte eucaryote en vue de la production de la protéine de transport membranaire procaryote fonctionnelle.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)