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1. (WO2019064242) MODULAR DNA ASSEMBLY SYSTEM
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Pub. No.: WO/2019/064242 International Application No.: PCT/IB2018/057527
Publication Date: 04.04.2019 International Filing Date: 28.09.2018
IPC:
C12N 15/66 (2006.01) ,C12N 15/70 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66
General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70
Vectors or expression systems specially adapted for E. coli
Applicants:
VAN DOLLEWEERD, Craig John [NZ/NZ]; NZ
VICTORIA LINK LIMITED [NZ/NZ]; Rankine Brown Building 21 Kelburn Parade Kelburn Wellington, 6012, NZ
Inventors:
VAN DOLLEWEERD, Craig John; NZ
PARKER, Emily Jane; NZ
NICHOLSON, Matthew Joseph; NZ
Agent:
BLUE PENGUIN IP LIMITED; NZ
Priority Data:
201790395529.09.2017AU
Title (EN) MODULAR DNA ASSEMBLY SYSTEM
(FR) SYSTÈME D'ASSEMBLAGE D'ADN MODULAIRE
Abstract:
(EN) A modular and hierarchical DNA assembly platform for synthetic biology is described. This enabling technology, termed MIDAS (for Modular Idempotent DNA Assembly System), can precisely assemble multiple DNA fragments in a single reaction using a standardised assembly design. It can be used to build genes from libraries of sequence-verified, reusable parts and to assemble multiple genes in a single vector. We describe the design and use of MIDAS, and its application in the reconstruction of the metabolic pathway for production of paspaline, a key intermediate in the biosynthesis of a range of indole diterpenes — a class of economically important secondary metabolites produced by several species of filamentous fungi.
(FR) L'invention concerne une plateforme d'assemblage d'ADN modulaire et hiérarchique pour la biologie synthétique. Cette technologie habilitante, appelée MIDAS (pour Système d'Assemblage d'ADN Modulaire Idempotent), peut assembler avec précision de multiples fragments d'ADN en une seule réaction à l'aide d'une conception d'ensemble standardisée. Elle peut être utilisée pour construire des gènes à partir de bibliothèques de parties à séquence vérifiée, réutilisables et pour assembler de multiples gènes dans un seul vecteur. Nous décrivons la conception et l'utilisation du MIDAS, et son application dans la reconstruction de la voie métabolique pour la production de paspaline, un intermédiaire clé dans la biosynthèse d'une gamme de diterpènes d'indole - une classe de métabolites secondaires économiquement importants produits par plusieurs espèces de champignons filamenteux.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)