Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2019064006) ERROR DETECTION DURING HYBRIDISATION OF TARGET DOUBLE-STRANDED NUCLEIC ACID
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2019/064006 International Application No.: PCT/GB2018/052753
Publication Date: 04.04.2019 International Filing Date: 27.09.2018
Chapter 2 Demand Filed: 15.03.2019
IPC:
C12Q 1/6834 (2018.01) ,C12N 15/10 (2006.01)
[IPC code unknown for C12Q 1/6834]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
EVONETIX LTD [GB/GB]; Suite 6 Science Village Chesterford Research Park Little Chesterford Essex CB10 1XL, GB
Inventors:
HAYES, Matthew James; GB
SANCHES-KUIPER, Raquel Maria; GB
BYGRAVE, Daniel Adrian; GB
Agent:
BERRYMAN, Robert; GB
Priority Data:
1715852.829.09.2017GB
1721441.220.12.2017GB
Title (EN) ERROR DETECTION DURING HYBRIDISATION OF TARGET DOUBLE-STRANDED NUCLEIC ACID
(FR) DÉTECTION D'ERREUR PENDANT L'HYBRIDATION D'ACIDE NUCLÉIQUE DOUBLE BRIN CIBLE
Abstract:
(EN) A series of hybridisations is performed for forming a target double-stranded nucleic acid from initial fragments, where each further hybridisation step hybridises the direct products of a pair of earlier hybridisation steps. For at least one further hybridisation step HF, both of the corresponding pair of earlier hybridisation steps HE comprise an error-detecting type of hybridisation step, which includes an error detecting operation to detect whether the hybridised fragments formed in the error-detecting type of hybridisation step HE comprise at least one erroneous hybridised fragment, and discarding at least part of the erroneous fragment to exclude it from a subsequent further hybridisation step. By detecting and removing erroneous fragments throughout a staged and controlled hybridisation process, erroneous fragments are prevented from diluting the pool of error-free fragments at each hybridisation step, to improve yield.
(FR) Selon la présente invention, une série d'hybridations est réalisée pour former un acide nucléique double brin cible à partir de fragments initiaux, chaque étape d'hybridation supplémentaire hybridant les produits directs d'une paire d'étapes d'hybridation précédentes. Pour au moins une étape d'hybridation supplémentaire HF, les deux paires correspondantes d'étapes d'hybridation précédentes HE comprennent un type de détection d'erreur d'étape d'hybridation, qui comprend une opération de détection d'erreur pour détecter si les fragments hybridés formés selon le type de détection d'erreur d'étape d'hybridation HE comprennent au moins un fragment hybridé erroné, et l'élimination d'au moins une partie du fragment erroné pour l'exclure d'une étape d'hybridation supplémentaire ultérieure. La détection et l'élimination de fragments erronés tout au long d'un processus par étapes et contrôlé d'hybridation empêchent les fragments erronés de diluer le groupe de fragments exempts d'erreur à chaque étape d'hybridation, afin d'améliorer le rendement.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)